ECTS
3 credits
Training Structure
College of Sciences
List of Courses
Choose 1 of 3
Biochemistry Techniques
3 creditsHealth: Key Issues
3 creditsBiological Investigations
3 credits
Biochemistry Techniques
Level of Education
2 years of post-secondary education
ECTS
3 credits
Training Structure
College of Sciences
This course unit builds on the Biochemistry S3 course unit. This course unit places greater emphasis on practical aspects. The principles of common biochemistry techniques (protein separation techniques, protein quantification by spectrophotometry, Western blot/ELISA, etc.) will be covered in lectures, followed by experiments related to these techniques during lab sessions. Students will be expected to interpret and analyze the experiments conducted during the lab sessions.
Health: Key Issues
Level of Education
2 years of post-secondary education
ECTS
3 credits
Training Structure
College of Sciences
This is a general education course that will cover a wide range of current topics related to human health. The course will address a diverse array of themes through 1.5-hour mini-seminars, taking both a pragmatic and critical approach. The course’s many guest speakers will share their expertise on topics such as immunity, molecular biology, cancer, nutrition, diagnosis, vaccination, bioethics, ecology, neuroscience, emerging diseases, and current and future therapeutic treatments. Each presentation will aim not only to provide cutting-edge knowledge and engage in a critical analysis of its subject matter, but also to guide students in researching and filtering scientific information to combat misinformation. Regarding the major challenges facing human health in the 21st century, we will address the real issues, the false controversies, and the solutions we can offer.
Biological Investigations
Level of Education
2 years of post-secondary education
ECTS
3 credits
Training Structure
College of Sciences
This unit is dedicated to biomarkers. It serves as an introduction to detection and diagnostic techniques. It covers various aspects of biomarker analysis:
Molecular markers / techniques for identification through genomic analysis in medicine and agronomy.
1) The Concept of Polymorphism and Detection Techniques: RFLP / ER-Nucleic Acid Probes
2) RFLP markers and other genetic markers: SNPs, STRs.
3) Search for new molecular markers: differential screening of cDNA libraries / subtractive libraries / transcriptomics
4) Other genomic analyses of polymorphism: AFLP / DNA fingerprinting.
Identification Techniques in the Agri-Food Industry Using Immunological Methods
1) Basic Concepts in Immunological Techniques
2) Agglutination Reactions
3) Enzyme-linked immunosorbent assay (ELISA) methods
Case studies of applications in the agri-food industry:
- study of the diagnostic kit for beet rhizominosis (sandwich ELISA)
- determination of ochratoxin A in cereals (competitive ELISA)
- assessment of fish freshness by histamine determination (competitive ELISA)
Biochemical identification of protein markers and other substances (metabolites)
1) Fundamentals of chromatography and the physical characterization of a spectrum (the interactions involved in each case and the solvents used to carry them out).
2) Affinity chromatography
2.1) Principle of this type of analysis
2.2) Finding the best tag for preparing a specific gel.
2.3) Their usefulness for various areas of research.
3) Study of protein-protein, protein-DNA, and other interactions…
4) HPLC, FPLC, and gas chromatography.