Biochemistry Techniques

This course unit builds on the Biochemistry S3 course unit. This course unit places greater emphasis on practical aspects. The principles of common biochemistry techniques (protein separation techniques, protein quantification by spectrophotometry, Western blot/ELISA, etc.) will be covered in lectures, followed by experiments related to these techniques during lab sessions. Students will be expected to interpret and analyze the experiments conducted during the lab sessions.

Knowledge: Understand the principles of the following techniques

- Principles of Protein Assays by Spectrophotometry

- Principles of gel exclusion chromatography, ion exchange, and affinity chromatography, CCM

- Principles of protein separation techniques (SDS-PAGE gel electrophoresis).

- Western blot and ELISA

-Skills: Be able to conduct experiments using the techniques discussed in class:

- Protein quantification by spectrophotometry (Bradford, Lowry, Biuret), standard curve, and quantification of protein concentration in a sample,

- Separation of amino acids by ion-exchange chromatography, identification of amino acids by CCM,

- Protein separation by SDS-PAGE electrophoresis 

- Preparation of several buffer solutions

-Interpersonal Skills: Be able to work well with others in pairs in the lab

- Work in pairs,

Using the workspace (workbenches and shared workspaces),

Communicate with teachers,

Adhere to the specified work hours,

Submit work by the deadline

Biochemistry, 3rd Semester