ECTS
2 credits
Component
Faculty of Science
Description
Understanding of screening techniques for bioactive molecules, and more generally in vitro tests used to measure a biological event in the perspective of drug discovery or diagnosis.
1) Pharmacological and biophysical fundamentals describing a biological event, target of biological tests:
2) Biological tests for the development of medicines or diagnostics
3) Applications, case studies, critical analyses.
Hourly volumes* :
CM : 15 H
TD : 5 H
Objectives
Be able to choose the biological screening and testing techniques suitable for the evaluation and optimisation of bioactive molecules.
Understand from a molecular point of view the technologies associated with diagnostic and screening tools and be able to design new chemical tools for this purpose.
Necessary pre-requisites
Basic biology and biochemistry
Knowledge control
Final written exam of 2 hours:
- Documents allowed: yes
- Non-graphic calculator allowed: yes
- Internet allowed: yes (but no interpersonal communication possible)
Syllabus
Course: Inductive pedagogy (problems based on real cases of development of active molecules. Inverted classroom. Votes and quizzes during sessions, case studies. Support(s) available on ENT (Moodle): Course documents, reference publications, examination records.
1) Fundamentals (3H)
- receptors, enzymes
- binding, basics of receptors phramacology
- biological activity measurment
2) Principle of Bioassays for drug development and diagnosis (6H)
-highthroughput vs high content
-Biological model : isolated biomolecules, cell-based assays, animal models including transgenic models
-luminescence (fluorescence, FRET, radioluminescence, SPA), radioactivity, UV
-viability, proliferation and adhesion assays (for medical devices).
3) Systems and devices :
-robots HTS and library management
-enzyme based reagents and assays
-surface interaction measurement (QCM/SPR)
- lateral flow and chromatographic assays
3) Applications, case studies and critical evalaution.
TD (5 H): Individual work, presentation of techniques and publications to be prepared before and presented during the session.
Case studies based on drug development, diagnosis tools and medical devices.
Additional information
Administrative contact(s):
Secretariat Master Chemistry
https://master-chimie.edu.umontpellier.fr/