Level of study
BAC +4
ECTS
2 credits
Component
Faculty of Science
Description
Knowledge of the latest mass spectrometry techniques for the qualitative analysis of organic molecules and biomolecules.
1) Description of Fundamentals (Ion Science and Technology):
- Ionization techniques
- Analysis techniques
- Tandem mass spectrometry (MS/MS)
- LC/MS and LC/MS/MS couplings
2) Application in the context of biomolecule analysis and organic chemistry reaction monitoring.
Hourly volumes* :
CM : 15 H
TD : 5 H
Objectives
Be able to determine the mass spectrometry techniques adapted to the analysis of an organic molecule and a biomolecule.
Understand and be able to analyze the strategy implemented to follow a given synthesis.
Necessary pre-requisites
Mass Spectrometry, L3 level:
Basic concepts (gas phase ion production/measurement, instrumental diagram)
Organic chemistry, L3 level:
Basic concepts of reactivity
Chemistry of Biomolecules, M1 level:
Knowledge of the various families (structures, properties)
Knowledge control
Final written exam of 2 hours:
- Authorized documents: no
- Non-graphic calculator allowed: yes
- Internet allowed : no
Syllabus
Course: Inductive (problematic) and deductive pedagogy, Support(s) available on ENT (Moodle): Course documents, TD documents, exam annals and reference publications.
- Reminder of the fundamental principles: (3 H)
- Production and transfer of ions in gas phase,
- Sensitivity and detection limit,
- Measurement accuracy and resolution.
- Ionization methods: (3 H)
- Ambient technologies,
- Desorption technologies.
- Methods of analysis: (3 H)
- Low resolution analyzers,
- High resolution analyzers.
- Tandem mass spectrometry: (3 H)
- Principle of MS/MS,
- Configuration of analyzers (dissociations in space, in time, iterative),
- Vibrational and electronic activation methods.
- Coupled techniques: (3 H)
- Principle of LC/MS,
- Principle of LC/MS/MS.
TD (5 H): Individual work, exercises to prepare before and during the session.
Case studies (interpretation of MS and MS/MS spectra of biomolecules, interpretation of LC/MS and LC/MS/MS data of reaction monitoring in organic chemistry/biomolecule chemistry).