Analysis of biomolecules by mass spectrometry

  • Study level

    BAC +4

  • ECTS

    2 credits

  • Component

    Faculty of Science

Description

Knowledge of the latest mass spectrometry techniques for the qualitative analysis of organic molecules and biomolecules.

 

1) Description of fundamental principles (Ion Science and Technology) :

- Ionization techniques

- Analysis techniques

- Tandem mass spectrometry (MS/MS)

- LC/MS and LC/MS/MS couplings

2) Application in biomolecule analysis and organic chemistry reaction monitoring.

Hourly volumes* :

CM: 15 H

TD: 5 H

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Objectives

Be able to determine the mass spectrometry techniques best suited to the analysis of an organic molecule and a biomolecule.

Understand and be able to analyze the strategy implemented to follow a given synthesis.

 

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Necessary prerequisites

Mass spectrometry, level L3 :

Basic concepts (production/measurement of ions in the gas phase, instrumental diagram)

Organic chemistry, L3 level :

Reactivity basics            

Biomolecule Chemistry, M1 level:

Knowledge of the various families (structures, properties)

 

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Knowledge control

Final written exam, 2 hours:

  • Authorized documents: no
  • Non-graphic calculator allowed: yes
  • Internet allowed: no
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Syllabus

Course: Inductive (problem-based) and deductive pedagogy, Support(s) available on ENT (Moodle): Course documents, TD documents, exam annals and reference publications.

  1. Fundamental principles: (3 H)

- Ion production and transfer in the gas phase,

- Sensitivity and detection limit,

- Measurement accuracy and resolution.

  1. Ionization methods: (3 H)

- Ambient technologies,

- Desorption technologies.

  1. Analytical methods: (3 H)

- Low-resolution analyzers,

- High-resolution analyzers.

  1. Tandem mass spectrometry: (3 H)

- Principle of MS/MS,

- Configuration of analyzers (dissociations in space, in time, iterative),

- Vibrational and electronic activation methods.

  1. Coupled techniques: (3 H)

- Principle of LC/MS,

- Principle of LC/MS/MS.

 

Tutorials (5 hours): Individual work, exercises to be prepared before and during the session.

Case studies (interpretation of MS and MS/MS spectra of biomolecules, interpretation of LC/MS and LC/MS/MS data for reaction monitoring in organic chemistry/biomolecular chemistry).

 

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