LC-MS, MS/MS

Knowledge of LC/MS and LC/MS/MS techniques for characterizing organic molecules and biomolecules in complex environments. 

Description of the instruments and acquisition methods that will be used in practical work.

1) Chromatography techniques in analytical mode coupled with mass spectrometry with ambient ionization:

• LC/MS instruments,
• LC/MS/MS instrumentation.

2) Coupled spectral data acquisition devices.

Hourly volumes*:

CM: 9 H

Field: 11 a.m.

Be able to interpret spectral data from LC/MS and LC/MS/MS couplings.

Understand how liquid chromatography systems combined with mass spectrometry work with ambient ionization techniques equipped with analyzer configurations in MS and MS/MS modes, and be able to optimize methods according to sample complexity.

Mass spectrometry, M1 level:           

Advanced concepts (gas phase ion production/measurement, ionization, analyzer and activation,

MS/MS mode analyzer configurations, low and high energy fragmentation).

Separative Methods, M1 level:  

Knowledge of various types of liquid chromatography

2-hour final written exam:

• Authorized documents: no
• Non-graphing calculator permitted: yes
• Internet access: no

Course: Inductive (problem-based) and deductive teaching methods, Materials available on the digital workspace (Moodle): Course documents, tutorial documents, past exam papers, and reference publications.

1. Conventional HPLC instrumentation (1.5 hours)

- Reverse phase,

- UV detection.

1. Conventional instrumentation in ambient MS (1.5 hours)

- Dynamic introduction, sources, and mass analyzers,

- Data acquisition (LC, UV, MS).

1. Conventional instrumentation in analyzer configuration for low-energy MS/MS experiments. (3 H)

- Dissociations in space and time,

- MS/MS acquisition modes (MS2, MSn, MRM, DDA, IDA, SWATH, etc.).

1. LC/MS coupling devices (1.5 hours)
2. LC/MS/MS coupling devices (1.5 hours)

Fieldwork (11 hours): 3 practical sessions on research tools.

Illustration of 3 acquisition modes:

• Implementation of mixture characterization using LC/MS with Q technology and method optimization (1 session)
• Implementation of mixture characterization using LC/MS/MS with QqTof technology (1 session)

- Implementation of Full Scan, SIR, and MRM acquisition methods with QqQ technology and comparison of results (1 session)

Administrative contact(s):

Master's Program in Chemistry Secretariat

https://master-chimie.edu.umontpellier.fr/