LC-MS, MS/MS

Knowledge of LC/MS and LC/MS/MS techniques for characterizing organic molecules and biomolecules in complex media. 

Description of the instruments and acquisition modes to be used in practical work.

1) Analytical chromatography-mass spectrometry techniques with ambient ionization:

• LC/MS instrumentation,
• LC/MS/MS instrumentation.

2) Coupled spectral data acquisition devices.

Hourly volumes* :

CM: 9 H

Field : 11 H

Be able to interpret spectral data from LC/MS and LC/MS/MS couplings.

Understand the operation of liquid chromatography systems associated with mass spectrometry with ambient ionization techniques equipped with MS and MS/MS mode analyzer configuration and be able to optimize methods according to sample complexity.

Mass spectrometry, level M1 :           

Advanced concepts (gas-phase ion production/measurement, ionization, analyzer and activation),

analyzer configurations in MS/MS mode, low- and high-energy fragmentation).

Separative Methods, level M1 :  

Knowledge of the various types of liquid chromatography

Final written exam, 2 hours:

• Authorized documents: no
• Non-graphic calculator allowed: yes
• Internet allowed: no

Course: Inductive (problem-based) and deductive pedagogy, Support(s) available on ENT (Moodle): Course documents, TD documents, exam annals and reference publications.

1. Conventional HPLC instrumentation (1.5 H)

- Reverse phase,

- UV detection.

1. Conventional instrumentation in ambient MS (1.5 H)

- Dynamic introduction, sources and mass analyzers,

- Data acquisition (LC, UV, MS).

1. Conventional instrumentation in analyzer configuration for low-energy MS/MS experiments. (3 H)

- Dissociations in space and time,

- MS/MS acquisition modes (MS2, MSn, MRM, DDA, IDA, SWATH, etc.).

1. LC/MS coupling devices (1.5 H)
2. LC/MS/MS coupling devices (1.5 H)

Fieldwork (11 H): 3 practical sessions on research tools.

Illustration of 3 acquisition modes:

• Implementation of LC/MS mixture characterization with Q technology and method optimization (1 session)
• Mixture characterization using LC/MS/MS with QqTof technology (1 session)

- Implementation of Full scan, SIR, MRM acquisition methods with QqQ technology and comparison of results (1 session)

Administrative contact(s) :

Secretariat Master Chemistry

https://master-chimie.edu.umontpellier.fr/