LC-MS, MS/MS

Knowledge of LC/MS and LC/MS/MS techniques for the characterization of organic molecules and biomolecules in complex media. 

Description of the instruments and acquisition modes that will be used in the lab.

1) Analytical Chromatography techniques coupled to mass spectrometry with ambient ionization:

• LC/MS type instrumentation,
• LC/MS/MS type instrumentation.

2) Coupled spectral data acquisition devices.

Hourly volumes* :

CM : 9 H

Field : 11 H

Be able to interpret spectral data from LC/MS and LC/MS/MS couplings.

Understand the operation of liquid chromatography systems associated with mass spectrometry with ambient ionization techniques equipped with MS and MS/MS mode analyzer configurations and be able to optimize methods according to sample complexity.

Mass spectrometry, level M1:           

Advanced concepts (gas phase ion production/measurement, ionization, analyzer and activation,

Analyzer configurations in MS/MS mode, low and high energy fragmentation).

Separative Methods, M1 level:  

Knowledge of the various types of liquid chromatography

Final written exam of 2 hours:

• Authorized documents: no
• Non-graphic calculator allowed: yes
• Internet allowed : no

Course: Inductive (problematic) and deductive pedagogy, Support(s) available on ENT (Moodle): Course documents, TD documents, exam annals and reference publications.

1. Conventional instrumentation in HPLC (1,5 H)

- Reverse phase,

- UV detection.

1. Conventional instrumentation in ambient MS (1.5 H)

- Dynamic introduction, sources and mass analyzers,

- Data acquisition (LC, UV, MS).

1. Conventional instrumentation in analyzer configuration for low energy MS/MS experiments. (3 H)

- Dissociations in space and time,

- MS/MS acquisition modes (MS2, MSn, MRM, DDA, IDA, SWATH, ...).

1. LC/MS coupling devices (1.5 H)
2. LC/MS/MS coupling devices (1.5 H)

Field (11 H): 3 sessions of practical work on research instruments.

Illustration of 3 acquisition modes:

• Implementation of the characterization of a mixture in LC/MS with Q technology and method optimization (1 session)
• Implementation of the characterization of a mixture in LC/MS/MS with QqTof technology (1 session)

- Implementation of Full scan, SIR, MRM acquisition methods with QqQ technology and comparison of results (1 session)

Administrative contact(s):

Secretariat Master Chemistry

https://master-chimie.edu.umontpellier.fr/