LC-MS, MS/MS

Knowledge of LC/MS and LC/MS/MS techniques for the characterization of organic molecules and biomolecules in complex matrices. 

Description of the instruments and data acquisition methods that will be used in the lab.

1) Analytical chromatography techniques coupled with mass spectrometry using ambient ionization:

• LC/MS-type instruments,
• LC/MS/MS-type instruments.

2) Coupled spectral data acquisition systems.

Hourly Volumes*:

CM: 9:00 a.m.

Land: 11 H

Be able to interpret spectral data from LC/MS and LC/MS/MS analyses.

Understand how liquid chromatography systems coupled with mass spectrometry—using ambient ionization techniques and equipped with analyzers configured for MS and MS/MS modes—operate, and be able to optimize methods based on sample complexity.

Mass Spectrometry, Level M1:           

Advanced Concepts (gas-phase ion generation and measurement, ionization, analyzers, and activation,

(MS/MS analyzer configurations, low- and high-energy fragmentation).

Separation Methods, M1 Level:  

Knowledge of the various types of liquid chromatography

2-hour final written exam:

• Authorized documents: No
• Non-graphical calculators allowed: yes
• Internet access allowed: No

Course: Inductive (problem-based) and deductive pedagogy. Materials available on the digital learning platform (Moodle): Course materials, tutorial materials, past exam questions, and reference publications.

1. Conventional HPLC Instrumentation (1.5 hours)

- Reverse phase,

- UV detection.

1. Conventional instrumentation in ambient MS (1.5 H)

- Dynamic introduction, mass sources, and mass analyzers,

- Data acquisition (LC, UV, MS).

1. Conventional instrumentation in an analyzer configuration for low-energy MS/MS experiments. (3 hours)

- Dissociations in space and time,

- MS/MS acquisition modes (MS2, MSn, MRM, DDA, IDA, SWATH, etc.).

1. LC/MS coupling systems (1.5 H)
2. LC/MS/MS coupling systems (1.5 H)

Fieldwork (11 hours): 3 practical sessions on research tools.

Illustration of 3 acquisition methods:

• Implementation of LC/MS characterization of a mixture using Q technology and method optimization (1 session)
• Implementation of Mixture Characterization Using LC/MS/MS with QqTof Technology (1 session)

- Implementation of full-scan, SIR, and MRM acquisition methods using QqQ technology, and comparison of the results (1 session)

Administrative contact(s):

Master's in Chemistry Office

https://master-chimie.edu.umontpellier.fr/