Extraction and separation of biomolecules

  • Level of education

    Master's degree

  • ECTS

    2 credits

  • Training structure

    Faculty of Science

Description

This course will cover techniques for extracting biomolecules (protein precipitation, SPE) and techniques for separating biomolecules (chromatography, electrophoresis).

Hourly volumes:

            CM: 9 a.m.

            Field: 11 a.m.

 

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Objectives

Acquire theoretical and practical knowledge of techniques for extracting and separating biomolecules. Know how to choose an extraction and separation technique based on the matrix and nature of the compounds to be studied and/or purified.

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Teaching hours

  • Extraction and separation of biomolecules - CMLecture9 a.m.

Knowledge assessment

Continuous assessment (practical work, 40%) and final assessment (lectures, 60%)

2 sessions for the final exam

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Syllabus

Regarding lectures: 7.5 hours will be devoted to methods of extraction and separation of biomolecules and 1.5 hours to teaching capillary electrophoresis.

Several practical sessions will illustrate these concepts: -

  • HPTLC applied to biomolecules
  • Capillary electrophoresis (chiral separation of amino acids),
  • SPE-HPLC biomolecules
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Additional information

Teaching team: Béatrice ROY, Joseph CHAMIEH, Isabelle LEFEBVRE-TOURNIER

 

Administrative contact(s):

Master's Program in Chemistry Secretariat

https://master-chimie.edu.umontpellier.fr/

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