Extraction and separation of biomolecules

This course will teach techniques for the extraction of biomolecules (protein precipitation, SPE), as well as techniques for the separation of biomolecules (chromatography, electrophoresis).

Hourly volumes* :

            CM : 9 h

            Field: 11 a.m.

Acquire theoretical and practical knowledge of techniques for the extraction and separation of biomolecules. To know how to choose an extraction and separation technique according to the matrix and the nature of the compounds to study and/or purify.

Continuous assessment (practical work, 40%) and final assessment (lectures, 60%)

2 sessions for the final test

Concerning the lectures: 7.5 hours will be devoted to the methods of extraction and separation of biomolecules and 1.5 hours to the teaching of capillary electrophoresis

Several practical sessions will illustrate these concepts: -

• HPTLC applied to biomolecules
• Capillary electrophoresis (chiral separation of aa),
• SPE- HPLC biomolecules

Teaching team: Béatrice ROY, Joseph CHAMIEH, Isabelle LEFEBVRE-TOURNIER

Administrative contact(s):

Secretariat Master Chemistry

https://master-chimie.edu.umontpellier.fr/