Screening

Understanding of screening techniques for bioactive molecules, and more generally in vitro tests used to measure a biological event in the context of drug discovery or diagnosis.

1) Pharmacological and biophysical fundamentals describing a biological event, target of biological tests:

2) Biological tests for the development of medicines or diagnostics

3) Applications, case studies, critical analyses.

Hourly volumes*:

CM: 3 p.m.

Tutorial: 5 hours

Be able to choose the biological screening and testing techniques suitable for the evaluation and optimization of bioactive molecules.

Understand from a molecular point of view the technologies associated with diagnostic and screening tools and be able to design new chemical tools for this purpose.

Basic concepts of biology and biochemistry          

2-hour final written exam:

• Authorized documents: yes
• Non-graphing calculator permitted: yes
• Internet access permitted: yes (but no interpersonal communication possible)

Course: Inductive pedagogy (problems based on real cases of development of active molecules. Inverted classroom. Votes and quizzes during sessions, case studies. Support(s) available on ENT (Moodle): Course documents, reference publications, examination records.

1) Fundamentals (3 hours)

- receptors, enzymes

- binding, basics of receptor pharmacology

- biological activity measurement

2) Principle of Bioassays for drug development and diagnosis (6H)

-high throughput vs high content

-Biological model: isolated biomolecules, cell-based assays, animal models including transgenic models

-luminescence (fluorescence, FRET, radioluminescence, SPA), radioactivity, UV

-viability, proliferation, and adhesion assays (for medical devices).

3) Systems and devices:

-HTS robots and library management

enzyme-based reagents and assays

-surface interaction measurement (QCM/SPR)

- lateral flow and chromatographic assays

3) Applications, case studies, and critical evaluation.

TD (5 H): Individual work, presentation of techniques and publications to be prepared before and presented during the session.

Case studies based on drug development, diagnostic tools, and medical devices.

Administrative contact(s):

Master's Program in Chemistry Secretariat

https://master-chimie.edu.umontpellier.fr/