Screening

Understanding of screening techniques for bioactive molecules, and, more generally, in vitro assays used to measure a biological event in the context of drug discovery or diagnosis.

1) Pharmacological and biophysical fundamentals describing a biological event that is the subject of biological tests:

2) Biological tests for the development of drugs or diagnostic tools

3) Applications, case studies, critical analyses.

Hourly Volumes*:

CM: 3:00 p.m.

TD: 5 hours

Be able to select the biological screening and testing techniques appropriate for the evaluation and optimization of bioactive molecules.

Understand, from a molecular perspective, the technologies associated with diagnostic and screening tools, and be able to design new chemical tools for this purpose.

Basic Concepts in Biology and Biochemistry          

2-hour final written exam:

• Authorized documents: yes
• Non-graphical calculators allowed: yes
• Internet access allowed: yes (but no interpersonal communication is possible)

Course: Inductive Pedagogy (problems based on real-world cases involving the development of active molecules; flipped classroom; polls and quizzes during sessions; case studies). Resources available on ENT (Moodle): Course materials, reference publications, exam records.

1) Fundamentals (3 hours)

- receptors, enzymes

- Binding, Basics of Receptor Pharmacology

- measurement of biological activity

2) Principles of Bioassays for Drug Development and Diagnosis (6H)

-high throughput vs. high content

-Biological model: isolated biomolecules, cell-based assays, animal models, including transgenic models

-luminescence (fluorescence, FRET, radioluminescence, SPA), radioactivity, UV

-viability, proliferation, and adhesion assays (for medical devices).

3) Systems and devices:

-HTS robots and library management

-enzyme-based reagents and assays

-surface interaction measurement (QCM/SPR)

- lateral flow and chromatographic assays

3) Applications, case studies, and critical evaluation.

TD (5 hours): Individual work; presentation of techniques and publications to be prepared in advance and presented during the session.

Case studies on drug development, diagnostic tools, and medical devices.

Administrative contact(s):

Master's in Chemistry Office

https://master-chimie.edu.umontpellier.fr/