Supported Syntheses

In chemistry, solid-phase synthesis is a method in which molecules are covalently bonded to a solid support and synthesized step by step using selective protective groups. The purpose of this applied course is to provide a comprehensive understanding of this field and to examine supported strategies for the practical preparation of polypeptides and oligonucleotides.

Hourly Volumes*:

CM: 9:00 a.m. 

Land: 11 H

Mastering the tools of supported-phase synthesis

 Organic Chemistry (M1 Level)

Final written exam, 1 hour and 30 minutes

Authorized documents: No

Non-graphical calculators allowed: yes

Internet access allowed: No

Practical assignments assessed in the form of a group report

Course:

The theoretical concepts covered will include:

1. The course will cover automated DNA and RNA synthesis (equipment), the various types of substrates that can be used, as well as specific separation and characterization methods (circular dichroism, half-melt temperature, gel electrophoresis, mass spectrometry, reverse-phase HPLC, and ion-exchange HPLC).
2. Supported synthesis in organic chemistry in general. The course focuses on the various types of equipment, the properties of different polymers used in supported synthesis depending on the medium and the chosen reagents, as well as the various mechanisms that allow the substrate to bind to the resin and the synthesized product to be cleaved.

Fieldwork: The concepts covered in the course will be illustrated through two hands-on group sessions.

A session will be held at the ChemBioNAC team’s facilities. Students will have access to the laboratory’s DNA synthesizers and will synthesize an oligonucleotide sequence and characterize it using MALDI-TOF and HPLC (reverse-phase and ion-polarity).

A session will take place at the SMART team’s facilities or in a lab, depending on room availability. Students will immobilize the periodate reagent on a macro-crosslinked resin that does not swell. The resin’s capacity will then be determined before it is used to carry out a Malaprade reaction, in which both the reagent and its byproduct will be retained on the resin, while the reaction product will remain in solution. During this session, a supported synthesis of a polypeptide will also be carried out in parallel, in which the growing substrate will be bound to a Merrifield-type resin.

Administrative contact(s):

Master's in Chemistry Office

https://master-chimie.edu.umontpellier.fr/