Supported Synthesis

In chemistry, solid phase synthesis is a method in which molecules are covalently bonded to a solid support and synthesized step by step using selective protecting groups. The purpose of this applied course is to provide a comprehensive understanding of this field and to examine supported strategies for the practical preparation of polypeptides and oligonucleotides.

Hourly volumes* :

CM : 9 H 

Field : 11 H

Mastering the tools of supported phase synthesis

 Organic chemistry level M1

Final written exam of 1h30

Authorized documents: no

Non-graphic calculator allowed: yes

Internet allowed : no

Practical work evaluated in the form of a group report

Course:

Theoretical concepts covered will include:

1. The automated synthesis of DNA and RNA (equipment), the different types of support that can be used as well as the methods of separation and characterization specifically dedicated (circular dichroism, half-melting temperature, gel electrophoresis, mass spectrometry, HPLC reverse phase and ionic) will be discussed.
2. Supported synthesis in organic chemistry in general. The course is focused on the different devices, the properties of the different polymers that can be used in supported synthesis according to the medium and the chosen reagents, as well as on the different links allowing the attachment of the substrate to the resin and the cleavage of the synthesized product

Field: The concepts presented in the course will be illustrated in the form of two practical sessions carried out in groups.

One session will be conducted in the ChemBioNAC team. Students will have access to the laboratory's DNA synthesizers and will carry out the synthesis of an oligonucleotide sequence as well as its characterization by MALDI-TOF and HPLC (reverse and ionic phase).

A session will be held in the SMART team's facilities or in a lab room depending on the availability of the facilities. Students will attach the periodate reagent to a macro-crosslinked resin that has no swelling properties. The capacity of the resin will then be determined, before being used to perform a Malaprade reaction, in which both the reagent and its by-product will be held on the resin, while the reaction product is in solution. In this session, a supported synthesis of a polypeptide will also be performed in parallel, in which the growing substrate will be bound to a Merrifield-type resin.

Administrative contact(s):

Secretariat Master Chemistry

https://master-chimie.edu.umontpellier.fr/