Study level
BAC +5
ECTS
2 credits
Component
Faculty of Science
Description
In chemistry, solid-phase synthesis is a method in which molecules are covalently bonded to a solid support and synthesized step by step using selective protecting groups. The aim of this applied course is to provide a comprehensive understanding of this field and to examine supported strategies for the practical preparation of polypeptides and oligonucleotides.
Hourly volumes* :
CM: 9 H
Field : 11 H
Objectives
Master the tools of supported phase synthesis
Necessary prerequisites
Organic chemistry level M1
Knowledge control
Final written exam (1h30)
Authorized documents: no
Non-graphic calculator allowed: yes
Internet allowed: no
Practical work assessed in the form of a group report
Syllabus
Course:
Theoretical concepts covered will include :
- We'll cover automated DNA and RNA synthesis (equipment), the different types of support that can be used, and specifically dedicated separation and characterization methods (circular dichroism, half-melting temperature, gel electrophoresis, mass spectrometry, reverse-phase and ionic HPLC).
- Supported synthesis in organic chemistry in general. The course focuses on the different types of apparatus, the properties of the different polymers used in supported synthesis depending on the medium and reagents chosen, and the different links that enable the substrate to be attached to the resin and the synthesized product to be cleaved.
Field: The concepts presented in the course will be illustrated in two practical group sessions.
One session will take place on the ChemBioNAC team's premises. Students will have access to the laboratory's DNA synthesizers and will synthesize an oligonucleotide sequence and characterize it by MALDI-TOF and HPLC (reverse and ionic phase).
One session will take place on the SMART team's premises or in a practical room, subject to availability. Students will attach the periodate reagent to a macro-crosslinked resin with no swelling properties. The capacity of the resin will then be determined, before being used to perform a Malaprade reaction, in which both the reagent and its by-product will be held on the resin, while the reaction product will be in solution. This session will also include a parallel supported polypeptide synthesis in which the growing substrate will be bound to a Merrifield-type resin.
Further information
Administrative contact(s) :
Secretariat Master Chemistry
https://master-chimie.edu.umontpellier.fr/