Supported syntheses

In chemistry, solid-phase synthesis is a method in which molecules are covalently bound to a solid support and synthesized step by step using selective protective groups. This applied course aims to provide a comprehensive understanding of this field and to examine the supported strategies that enable the practical preparation of polypeptides and oligonucleotides.

Hourly volumes*:

CM: 9 H 

Field: 11 a.m.

Mastering supported phase synthesis tools

 Organic chemistry, M1 level

Final written exam lasting 1 hour 30 minutes

Authorized documents: no

Non-graphing calculator permitted: yes

Internet access: no

Practical work assessed in the form of a group report

Course:

The theoretical concepts covered will include:

1. Topics covered will include automated DNA and RNA synthesis (equipment), the different types of media that can be used, and specific separation and characterization methods (circular dichroism, melting temperature, gel electrophoresis, mass spectrometry, reverse-phase HPLC, and ion chromatography).
2. Supported synthesis in organic chemistry in general. The course focuses on the different equipment, the properties of the different polymers that can be used in supported synthesis depending on the medium and reagents chosen, as well as the different bonds that allow the substrate to attach to the resin and then the cleavage of the synthesized product.

Fieldwork: The concepts covered in the course will be illustrated in the form of two practical sessions carried out in groups.

A session will be held at the ChemBioNAC team's premises. Students will have access to the laboratory's DNA synthesizers and will synthesize an oligonucleotide sequence and characterize it using MALDI-TOF and HPLC (reverse phase and ionic).

A session will take place in the SMART team's premises or in a practical work room, depending on availability. Students will fix the periodate reagent to a macro-crosslinked resin with no swelling properties. The capacity of the resin will then be determined before it is used to perform a Malaprade reaction, in which both the reagent and its by-product will be held on the resin, while the reaction product will be in solution. During this session, a supported synthesis of a polypeptide will also be performed in parallel, in which the growing substrate will be bound to a Merrifield-type resin.

Administrative contact(s):

Master's Program in Chemistry Secretariat

https://master-chimie.edu.umontpellier.fr/