Mass Spectrometry

Knowledge of the latest mass spectrometry techniques for the detection, identification, and structural characterization of organic molecules and biomolecules.

Applications in chemistry (pharmaceutical industry) and biology (omics approaches).

1) Structural elucidation (Ion characterization technologies):

• LC/MS/MS and exact mass measurements 
• Isotope Labeling, H/D Exchange
• Ionic Mobility

2) Surface analysis and imaging (molecular mapping)

3) Applications in chemistry and biology: characterization of small organic molecules and biomolecules.

Hourly Volumes*:

CM: 3:00 p.m.

TD: 5 hours

Be able to determine the structure of a small organic molecule and a biomolecule using mass spectrometry.

Understand and be able to analyze the strategy used to characterize an organic compound and a biomolecule in a complex sample.

Understand the implementation and areas of application of imaging-based analyses.

Mass Spectrometry, Level M1:           

Advanced Concepts (Gas-Phase Ion Generation and Measurement, Ionization, Analyzers, and Activation)

2-hour final written exam:

• Authorized documents: No
• Non-graphical calculators allowed: yes
• Internet access allowed: No

Course: Inductive (problem-based) and deductive pedagogy. Materials available on the digital learning platform (Moodle): Course materials, tutorial materials, past exam questions, and reference publications.

1. Identification Methodology: (3 hours)

- Analysis in complex matrices (LC/MS),

- High-resolution and accurate mass spectrometry (HR-MS),

- Analysis of discrete molecules and polymers.

1. Methodology for Structural Characterization: (6 hours)

- Fragmentation (MS/MS) and low- and high-energy dissociation (activation methods),

- Isotopic labeling and H/D exchange,

- Principle of ion mobility (IM/MS).

1. Multispectral Imaging (MSI) Analysis: (3 hours)

- Principle of surface analysis,

- Principles of Imaging and Instrumentation.

1. Applications in Chemistry and Biology: (3 hours)

- Spectral analysis (software tools and databases),

- Analysis of biomolecules (peptides, proteins, lipids, DNA, sugars, etc.),

- Polymer analysis (polydisperse samples, PEG),

- Omics Analyses (Background and Applications).

TD (5 hours): Individual work; exercises to be prepared before and during the session.

Case Studies:

- Control/Monitoring of organic synthesis,

- Chemical library screening,

- Targeted detection of compounds of interest,

- Structural determination of a small organic molecule,

- Examples of analyses of biomolecules and polymers,

- Spectral analysis in IM-MS and MSI modes.

Administrative contact(s):

Master's in Chemistry Office

https://master-chimie.edu.umontpellier.fr/