Functionalization/Bioconjugation

Bioconjugation reactions are of major interest in the biomedical sciences and allow chemists to modify biomolecules to confer new functions or properties. In this course, bioconjugation and biomolecule labeling strategies will be discussed in order to explore complex biological systems. The practical sessions will illustrate these concepts through examples of bioconjugation of osidic and nucleotidic platforms.

Hourly volumes* :

CM : 9 H 

Field : 11 H

The objective of this course is to provide the student with theoretical and practical knowledge in the field of bioconjugation reactions and their implementation techniques

Organic chemistry level M1

Final written exam of 1h30

Authorized documents: no

Non-graphic calculator allowed: yes

Internet allowed : no

Practical work evaluated in the form of a group report

Course: 9H

1. Functional target: Modification of sugars, polysaccharides and glycoconjugates - modification of nucleic acids and oligonucleotides.

1. Chemical modifications of biomolecules, Reactivity of the main functional groups (amine, thiol, carboxylate, azide etc...) of biomolecules. Bioorthogonal ligation reactions.

1. Bioconjugation reagents: homobifunctional, heterobifunctional and trifunctional links; self-immolating probes and tags.

Field: 11H

The practical sessions (2 x 5.5 H) carried out in groups will aim to illustrate the concepts detailed in the course. A first session will be dedicated to the synthesis of a small functional molecule that will be introduced into a target biomolecule (polysaccharide or oligonucleotide) in a second session. The new compounds synthesized during these practical sessions will also be tested to evaluate their biological activity. Students will be directly involved in an existing research project.

Administrative contact(s):

Secretariat Master Chemistry

https://master-chimie.edu.umontpellier.fr/