Functionalization/Bioconjugation

Bioconjugation reactions are of major interest in the biomedical sciences and enable chemists to modify biomolecules to impart new functions or properties to them. This course will cover strategies for bioconjugation and the labeling of biomolecules, which allow for the exploration of complex biological systems. The lab sessions will illustrate these concepts through examples of bioconjugation using osidic and nucleotide platforms.

Hourly Volumes*:

CM: 9:00 a.m. 

Land: 11 H

The objective of this course unit is to provide students with theoretical and practical knowledge in the field of bioconjugation reactions and the techniques used to carry them out.

Organic Chemistry (M1 Level)

Final written exam, 1 hour and 30 minutes

Authorized documents: No

Non-graphical calculators allowed: yes

Internet access allowed: No

Practical assignments assessed in the form of a group report

Class: 9H

1. Functional target: Modification of sugars, polysaccharides, and glycoconjugates—modification of nucleic acids and oligonucleotides.

1. Chemical modifications of biomolecules; reactivity of the main functional groups (amine, thiol, carboxylate, azide, etc.) in biomolecules; bioorthogonal ligation reactions.

1. Bioconjugation reagents: Homobifunctional, heterobifunctional, and trifunctional linkers; self-immolating probes and tags.

Land: 11H

The laboratory sessions (2 x 5.5 hours), conducted in groups, will aim to illustrate the concepts covered in class. The first session will be devoted to the synthesis of a small functional molecule, which will be incorporated into a target biomolecule (polysaccharide or oligonucleotide) during the second session. The new compounds synthesized during these lab sessions will also be tested to evaluate their biological activity. Students will therefore be directly involved in an existing research project.

Administrative contact(s):

Master's in Chemistry Office

https://master-chimie.edu.umontpellier.fr/