Functionalization/Bioconjugation

Bioconjugation reactions are of major interest in the biomedical sciences, enabling chemists to modify biomolecules to confer new functions or properties. This course covers bioconjugation and biomolecule labeling strategies for exploring complex biological systems. Practical sessions will illustrate these concepts through examples of bioconjugation of osidic and nucleotide platforms.

Hourly volumes* :

CM: 9 H 

Field : 11 H

The objective of this UE is to provide theoretical and practical knowledge to the student in the field of bioconjugation reactions and their implementation techniques.

Organic chemistry level M1

Final written exam (1h30)

Authorized documents: no

Non-graphic calculator allowed: yes

Internet allowed: no

Practical work assessed in the form of a group report

Course : 9H

1. Functional target: Modification of sugars, polysaccharides and glycoconjugates - modification of nucleic acids and oligonucleotides.

1. Chemical modifications of biomolecules, Reactivity of the main functional groups (amine, thiol, carboxylate, azide etc.) of biomolecules. Bioorthogonal ligation reactions.

1. Bioconjugation reagents: Homobifunctional, Heterobifunctional and Trifunctional bonds; self-immolating probes and tags.

Land : 11H

The practical work sessions (2 x 5.5 H) carried out in groups will aim to illustrate the detailed concepts in class. A first session will be dedicated to the synthesis of a small functional molecule that will be introduced into a target biomolecule (polysaccharide or oligonucleotide) in a second session. The new compounds synthesized during these practical work sessions will also be tested to evaluate their biological activity. Students will therefore be directly involved in an existing research project.

Administrative contact(s) :

Secretariat Master Chemistry

https://master-chimie.edu.umontpellier.fr/