Extraction and Separation of Biomolecules

  • Level of Education

    5 years of post-secondary education

  • ECTS

    2 credits

  • Training Structure

    College of Sciences

Description

This course unit will cover techniques for the extraction of biomolecules (protein precipitation, SPE), as well as techniques for the separation of biomolecules (chromatography, electrophoresis).

Hourly volumes*:

            CM: 9 a.m.

            Field: 11 h

 

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Objectives

Acquire theoretical and practical knowledge of techniques for the extraction and separation of biomolecules. Be able to select an extraction and separation technique based on the matrix and the nature of the compounds to be studied and/or purified.

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Class Hours

  • Extraction and Separation of Biomolecules - LectureLecture9:00 a.m.

Knowledge Assessment

Continuous assessment (Practical Work, 40%) and final assessment (Classwork, 60%)

2 sessions for the final exam

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Course Outline

Regarding lectures: 7.5 hours will be devoted to methods for the extraction and separation of biomolecules, and 1.5 hours to teaching capillary electrophoresis

Several hands-on sessions will be held to illustrate these concepts: -

  • HPTLC Applied to Biomolecules
  • Capillary electrophoresis (chiral separation of amino acids),
  • SPE-HPLC for Biomolecules
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Additional Information

Faculty: Béatrice ROY, Joseph CHAMIEH, Isabelle LEFEBVRE-TOURNIER

 

Administrative contact(s):

Master's in Chemistry Office

https://master-chimie.edu.umontpellier.fr/

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