Biotechnology and applications

This course aims to highlight biological processes at the cellular level or even in living subjects. Different approaches to molecular imaging will be discussed (fluorescent probes, radiolabeling).

Hourly volumes*:

            CM: 9 a.m.

           Field: 11 a.m.

Be able to select the appropriate tools for detecting and visualizing biological targets.

Biomolecule Chemistry Level M1

2-hour final written exam:

• Authorized documents: no
• Non-graphing calculator permitted: yes
• Internet access: no

Course:

Absorption of UV-visible light; fluorescence; single-photon or two-photon lasers; radiolabeling; fluorescent probes.

Use of radiolabeling to highlight biological targets. Synthesis methods used.

Presentation of innovative strategies currently used for the fluorescent labeling of peptides and proteins. The focus will be on methods such as the introduction of a fluorescent amino acid into a peptide sequence, conjugation reactions with a fluorescent prosthetic group, and the main applications of GFP (Green Fluorescent Protein).

TP:

Fluorescence imaging experiments on living cells incubated with a fluorescent compound. Photodynamic therapy experiments in which students incubate cells in culture with nanoparticles or photoactivatable compounds.

Teaching staff :

Emmanuelle Remond

Magali Gary-Bobo