Study level
BAC +5
ECTS
2 credits
Component
Faculty of Science
Description
The aim of this course is to highlight biological processes at the cellular level or even in living subjects. Various molecular imaging approaches will be discussed (fluorescent probes, radiolabeling).
Hourly volumes* :
CM: 9 H
Field : 11 H
Objectives
Be able to choose the right tools for detecting and visualizing biological targets.
Necessary prerequisites
Chemistry of biomolecules level M1
Knowledge control
Final written exam, 2 hours:
- Authorized documents: no
- Non-graphic calculator allowed: yes
- Internet allowed: no
Syllabus
Course:
UV-visible light absorption; fluorescence; single-photon or two-photon lasers; radiolabeling; fluorescent probes.
Use of radiolabeling to identify biological targets. Synthesis methods involved.
Innovative strategies for fluorescent labeling of peptides and proteins. Emphasis will be placed on methods such as the introduction of a fluorescent amino acid into a peptide sequence, conjugation reactions with a fluorescent prosthetic group, and the main applications of GFP (Green Fluorescent Protein).
TP:
Fluorescence imaging experiments on living cells incubated with a fluorescent compound. Photodynamic therapy experiments in which students incubate cultured cells with photoactivatable nanoparticles or compounds.
Further information
Teaching team :
Emmanuelle Remond
Magali Gary-Bobo