Level of study
BAC +5
ECTS
2 credits
Component
Faculty of Science
Description
The aim of this course is to highlight biological processes at the cellular level or even in living subjects. Different molecular imaging approaches will be discussed (fluorescent probes, radiolabeling).
Hourly volumes* :
CM : 9 H
Field : 11 H
Objectives
Be able to choose the appropriate tools for the detection and visualization of biological targets.
Necessary pre-requisites
Chemistry of biomolecules level M1
Knowledge control
Final written exam of 2 hours:
- Authorized documents: no
- Non-graphic calculator allowed: yes
- Internet allowed : no
Syllabus
Course:
UV-visible light absorption; fluorescence; single or dual photon lasers; radiolabeling; fluorescent probes.
Use of radiolabeling for the detection of biological targets. Synthesis methods involved.
Presentation of innovative strategies used today for fluorescent labeling of peptides and proteins. Emphasis will be put on methods such as the introduction of a fluorescent amino acid in a peptide sequence, conjugation reactions with a fluorescent prosthetic group and the main applications of GFP (Green Fluorescent Protein).
TP:
Fluorescence imaging experiments on live cells incubated with a fluorescent compound. Photodynamic therapy experiments in which students incubate cells in culture with photoactivatable nanoparticles or compounds.
Additional information
Teaching team :
Emmanuelle Remond
Magali Gary-Bobo