Biotechnology and Applications

The purpose of this course is to highlight biological processes at the cellular level or even in living organisms. Various molecular imaging approaches will be covered (fluorescent probes, radiolabeling).

Hourly Volumes*:

            CM: 9:00 a.m.

           Land: 11 H

Be able to select the appropriate tools for detecting and visualizing biological targets.

Biomolecular Chemistry (M1 Level)

2-hour final written exam:

• Authorized documents: No
• Non-graphical calculators allowed: yes
• Internet access allowed: No

Course:

Absorption of UV-visible light; fluorescence; single-photon or two-photon lasers; radiolabeling; fluorescent probes.

Use of radiolabeling to identify biological targets. Synthesis methods involved.

An overview of innovative strategies currently used for the fluorescent labeling of peptides and proteins. The focus will be on methods such as the incorporation of a fluorescent amino acid into a peptide sequence, conjugation reactions with a fluorescent prosthetic group, and the main applications of GFP (Green Fluorescent Protein).

Practical Work:

Fluorescence imaging experiments on live cells incubated with a fluorescent compound. Photodynamic therapy experiments in which students incubate cultured cells with nanoparticles or photoactivatable compounds.

Teaching Staff :

Emmanuelle Remond

Magali Gary-Bobo