Mass spectrometry

Knowledge of the latest mass spectrometry techniques for the detection, identification and structural characterization of organic molecules and biomolecules.

Applications in chemistry (pharmaceutical industry) and biology (Omics approaches).

1) Structural elucidation (Ion characterization technologies):

• LC/MS/MS and exact mass measurements 
• Isotopic labelling, H/D exchange
• Ionic mobility

2) Surface analysis and imaging (molecular mapping)

3) Application in chemistry and biology: characterization of small organic molecules and biomolecules.

Hourly volumes* :

CM : 15 H

TD : 5 H

Be able to determine the structure of a small organic molecule and a biomolecule by mass spectrometry.

Understand and be able to analyze the strategy implemented to characterize an organic compound and a biomolecule in a complex sample.

Understand the implementation and application areas of imaging mode analysis.

Mass spectrometry, level M1:           

Advanced concepts (gas phase ion production/measurement, ionization, analyzer and activation)

Final written exam of 2 hours:

• Authorized documents: no
• Non-graphic calculator allowed: yes
• Internet allowed : no

Course: Inductive (problematic) and deductive pedagogy, Support(s) available on ENT (Moodle): Course documents, TD documents, exam annals and reference publications.

1. Identification Methodology: (3 H)

- Analysis in complex media (LC/MS),

- High resolution and exact mass measurement (HR-MS),

- Analysis of discrete molecules and polymers.

1. Structural characterization methodology: (6 H)

- Fragmentations (MS/MS) and low and high energy dissociation (activation methods),

- Isotopic labelling and H/D exchange,

- Principle of ion mobility (IM/MS).

1. Analysis in imaging mode (MSI): (3 H)

- Principle of surface analysis,

- Principle of imaging and instrumentation.

1. Applications in chemistry and biology: (3 H)

- Spectral interpretation (software tools and databases),

- Analysis of biomolecules (peptides, proteins, lipids, DNA, sugars, ...),

- Polymer analysis (polydisperse samples, PEG),

- Omics analysis (context and applications).

TD (5 H): Individual work, exercises to prepare before and during the session.

Case studies:

- Control/Follow-up of organic synthesis,

- Chemical library screening,

- Targeted detection of compounds of interest,

- Structural determination of a small organic molecule,

- Examples of biomolecule and polymer analysis,

- Spectral interpretation in IM-MS and MSI mode.

Administrative contact(s):

Secretariat Master Chemistry

https://master-chimie.edu.umontpellier.fr/