Mass spectrometry

Knowledge of the latest mass spectrometry techniques for the detection, identification, and structural characterization of organic molecules and biomolecules.

Applications in chemistry (pharmaceutical industry) and biology (Omics approaches).

1) Structural elucidation (ion characterization technologies):

• LC/MS/MS and accurate mass measurements 
• Isotopic labeling, H/D exchange
• Ionic mobility

2) Surface analysis and imaging (molecular mapping)

3) Application in chemistry and biology: characterization of small organic molecules and biomolecules.

Hourly volumes*:

CM: 3 p.m.

Tutorial: 5 hours

Be able to determine the structure of a small organic molecule and a biomolecule using mass spectrometry.

Understand and be able to analyze the strategy implemented to characterize an organic compound and a biomolecule in a complex sample.

Understanding the implementation and areas of application of imaging mode analyses.

Mass spectrometry, M1 level:           

Advanced concepts (gas phase ion production/measurement, ionization, analyzer, and activation)

2-hour final written exam:

• Authorized documents: no
• Non-graphing calculator permitted: yes
• Internet access: no

Course: Inductive (problem-based) and deductive teaching methods, Materials available on the digital workspace (Moodle): Course documents, tutorial documents, past exam papers, and reference publications.

1. Identification methodology: (3 hours)

- Analysis in complex environments (LC/MS),

- High resolution and accurate mass measurement (HR-MS),

- Analysis of discrete molecules and polymers.

1. Structural characterization methodology: (6 hours)

- Fragmentation (MS/MS) and low- and high-energy dissociation (activation methods),

- Isotopic labeling and H/D exchange,

- Principle of ion mobility (IM/MS).

1. Imaging mode analysis (MSI): (3 hours)

- Principle of surface analysis,

- Imaging principles and instrumentation.

1. Applications in chemistry and biology: (3 hours)

- Spectral interpretation (software tools and databases),

- Analysis of biomolecules (peptides, proteins, lipids, DNA, sugars, etc.),

- Polymer analysis (polydisperse samples, PEG),

- Omics analyses (context and applications).

TD (5 hours): Individual work, exercises to be prepared before and during the session.

Case studies:

- Control/Monitoring of organic synthesis,

- Chemical library screening,

- Targeted detection of compounds of interest,

- Structural determination of a small organic molecule,

- Examples of biomolecule and polymer analysis,

- Spectral interpretation in IM-MS and MSI modes.

Administrative contact(s):

Master's Program in Chemistry Secretariat

https://master-chimie.edu.umontpellier.fr/