Analysis of volatile molecules

Knowledge of gas chromatography and electron impact ionization mass spectrometry and quadrupole mass analyzer techniques for the analysis of volatile organic molecules.

1) GC-MS analyses of volatile organic compounds:

• Electron impact ionization (EI) techniques
• Chemical ionization (CI) techniques
• Quadrupole analysis techniques (Q)
• GC/MS Couplings

2) Applications in organic chemistry analysis, characterization of volatile samples.

Hourly volumes* :

CM : 15 H

TD : 5 H

Understand the mass spectrometry techniques coupled with gas chromatography (GC-MS) adapted to the analysis of a volatile organic molecule.

Be able to interpret GC-MS data.

Understand and be able to analyze the strategy implemented to characterize a vaporizable sample.

Mass Spectrometry, L3 level:

Basic concepts (gas phase ion production/measurement, instrumental diagram)

Final written exam of 2 hours:

• Authorized documents: no
• Non-graphic calculator allowed: yes
• Internet allowed : no

Course: Inductive (problematic) and deductive pedagogy, Support(s) available on ENT (Moodle): Course documents, TD documents, exam annals and reference publications.

Description of conventional mass spectrometry techniques coupled with gas chromatography for the qualitative analysis of volatile organic molecules.

1. Gas chromatography (3H)

- Principle,

- FID detection,

- Detection by mass spectrometry.

1. Hard ionization methods: (6H)

• Electronic Impact (EI):

-Production of molecular and fragment ions,

-Description of the main fragmentation families.

• Chemical ionization (CI).

1. Methods of analysis: (3H)

• Measurement accuracy and elemental composition,
• Low resolution quadrupole analyzer.

1. Coupled technique: (3H)

- Principle of GC/MS,

- Data acquisition in GC/MS.

TD (5 H): Individual work, exercises to prepare before and during the session.

Case studies with illustrations in various fields of application (interpretation of EI-MS and GC/EI-MS spectra of volatile organic molecules).