Level of study
BAC +4
ECTS
2 credits
Component
Faculty of Science
Description
Knowledge of gas chromatography and electron impact ionization mass spectrometry and quadrupole mass analyzer techniques for the analysis of volatile organic molecules.
1) GC-MS analyses of volatile organic compounds:
- Electron impact ionization (EI) techniques
- Chemical ionization (CI) techniques
- Quadrupole analysis techniques (Q)
- GC/MS Couplings
2) Applications in organic chemistry analysis, characterization of volatile samples.
Hourly volumes* :
CM : 15 H
TD : 5 H
Objectives
Understand the mass spectrometry techniques coupled with gas chromatography (GC-MS) adapted to the analysis of a volatile organic molecule.
Be able to interpret GC-MS data.
Understand and be able to analyze the strategy implemented to characterize a vaporizable sample.
Necessary pre-requisites
Mass Spectrometry, L3 level:
Basic concepts (gas phase ion production/measurement, instrumental diagram)
Knowledge control
Final written exam of 2 hours:
- Authorized documents: no
- Non-graphic calculator allowed: yes
- Internet allowed : no
Syllabus
Course: Inductive (problematic) and deductive pedagogy, Support(s) available on ENT (Moodle): Course documents, TD documents, exam annals and reference publications.
Description of conventional mass spectrometry techniques coupled with gas chromatography for the qualitative analysis of volatile organic molecules.
- Gas chromatography (3H)
- Principle,
- FID detection,
- Detection by mass spectrometry.
- Hard ionization methods: (6H)
- Electronic Impact (EI):
-Production of molecular and fragment ions,
-Description of the main fragmentation families.
- Chemical ionization (CI).
- Methods of analysis: (3H)
- Measurement accuracy and elemental composition,
- Low resolution quadrupole analyzer.
- Coupled technique: (3H)
- Principle of GC/MS,
- Data acquisition in GC/MS.
TD (5 H): Individual work, exercises to prepare before and during the session.
Case studies with illustrations in various fields of application (interpretation of EI-MS and GC/EI-MS spectra of volatile organic molecules).