Analysis of biomolecules by mass spectrometry

Knowledge of the latest mass spectrometry techniques for the qualitative analysis of organic molecules and biomolecules.

1) Description of Fundamentals (Ion Science and Technology):

- Ionization techniques

- Analysis techniques

- Tandem mass spectrometry (MS/MS)

- LC/MS and LC/MS/MS couplings

2) Application in the context of biomolecule analysis and organic chemistry reaction monitoring.

Hourly volumes* :

CM : 15 H

TD : 5 H

Be able to determine the mass spectrometry techniques adapted to the analysis of an organic molecule and a biomolecule.

Understand and be able to analyze the strategy implemented to follow a given synthesis.

Mass Spectrometry, L3 level:

Basic concepts (gas phase ion production/measurement, instrumental diagram)

Organic chemistry, L3 level:

Basic concepts of reactivity            

Chemistry of Biomolecules, M1 level:

Knowledge of the various families (structures, properties)

Final written exam of 2 hours:

• Authorized documents: no
• Non-graphic calculator allowed: yes
• Internet allowed : no

Course: Inductive (problematic) and deductive pedagogy, Support(s) available on ENT (Moodle): Course documents, TD documents, exam annals and reference publications.

1. Reminder of the fundamental principles: (3 H)

- Production and transfer of ions in gas phase,

- Sensitivity and detection limit,

- Measurement accuracy and resolution.

1. Ionization methods: (3 H)

- Ambient technologies,

- Desorption technologies.

1. Methods of analysis: (3 H)

- Low resolution analyzers,

- High resolution analyzers.

1. Tandem mass spectrometry: (3 H)

- Principle of MS/MS,

- Configuration of analyzers (dissociations in space, in time, iterative),

- Vibrational and electronic activation methods.

1. Coupled techniques: (3 H)

- Principle of LC/MS,

- Principle of LC/MS/MS.

TD (5 H): Individual work, exercises to prepare before and during the session.

Case studies (interpretation of MS and MS/MS spectra of biomolecules, interpretation of LC/MS and LC/MS/MS data of reaction monitoring in organic chemistry/biomolecule chemistry).