Analysis of Biomolecules by Mass Spectrometry

Knowledge of the latest mass spectrometry techniques for the qualitative analysis of organic molecules and biomolecules.

1) Description of the fundamental principles (Ion Science and Technology):

- Ionization Techniques

- Analytical Techniques

- Tandem mass spectrometry (MS/MS)

- LC/MS and LC/MS/MS couplings

2) Application in the analysis of biomolecules and the monitoring of organic chemical reactions.

Hourly Volumes*:

CM: 3:00 p.m.

TD: 5 hours

Be able to determine the mass spectrometry techniques appropriate for analyzing an organic molecule and a biomolecule.

Understand and be able to analyze the strategy used to track a given synthesis.

Mass Spectrometry, Level L3:

Basics (gas-phase ion generation and measurement, instrument diagram)

Organic Chemistry, 3rd-year level:

Basics of Reactivity            

Biomolecular Chemistry, M1 Level:

Knowledge of the various families (structures, properties)

2-hour final written exam:

• Authorized documents: No
• Non-graphical calculators allowed: yes
• Internet access allowed: No

Course: Inductive (problem-based) and deductive pedagogy. Materials available on the digital learning platform (Moodle): Course materials, tutorial materials, past exam questions, and reference publications.

1. Review of Fundamental Principles: (3 hours)

- Ion production and transport in the gas phase,

- Sensitivity and detection limit,

- Measurement accuracy and resolution.

1. Ionization methods: (3 H)

- Ambient technologies,

- Desorption technologies.

1. Analytical Methods: (3 hours)

- Low-resolution analyzers,

- High-resolution analyzers.

1. Tandem mass spectrometry: (3 H)

- The principle of MS/MS,

- Analyzer configuration (spatial, temporal, and iterative decoupling),

- Vibrational and electronic activation methods.

1. Combined Techniques: (3 hours)

- The principle of LC/MS,

- The Principle of LC/MS/MS.

TD (5 hours): Individual work; exercises to be prepared before and during the session.

Case studies (interpretation of MS and MS/MS spectra of biomolecules; interpretation of LC/MS and LC/MS/MS data from reaction monitoring in organic chemistry and biomolecular chemistry).