Analysis of biomolecules by mass spectrometry

Knowledge of the latest mass spectrometry techniques for the qualitative analysis of organic molecules and biomolecules.

1) Description of fundamental principles (Ion Science and Technology) :

- Ionization techniques

- Analysis techniques

- Tandem mass spectrometry (MS/MS)

- LC/MS and LC/MS/MS couplings

2) Application in biomolecule analysis and organic chemistry reaction monitoring.

Hourly volumes* :

CM: 15 H

TD: 5 H

Be able to determine the mass spectrometry techniques best suited to the analysis of an organic molecule and a biomolecule.

Understand and be able to analyze the strategy implemented to follow a given synthesis.

Mass spectrometry, level L3 :

Basic concepts (production/measurement of ions in the gas phase, instrumental diagram)

Organic chemistry, L3 level :

Reactivity basics            

Biomolecule Chemistry, M1 level:

Knowledge of the various families (structures, properties)

Final written exam, 2 hours:

• Authorized documents: no
• Non-graphic calculator allowed: yes
• Internet allowed: no

Course: Inductive (problem-based) and deductive pedagogy, Support(s) available on ENT (Moodle): Course documents, TD documents, exam annals and reference publications.

1. Fundamental principles: (3 H)

- Ion production and transfer in the gas phase,

- Sensitivity and detection limit,

- Measurement accuracy and resolution.

1. Ionization methods: (3 H)

- Ambient technologies,

- Desorption technologies.

1. Analytical methods: (3 H)

- Low-resolution analyzers,

- High-resolution analyzers.

1. Tandem mass spectrometry: (3 H)

- Principle of MS/MS,

- Configuration of analyzers (dissociations in space, in time, iterative),

- Vibrational and electronic activation methods.

1. Coupled techniques: (3 H)

- Principle of LC/MS,

- Principle of LC/MS/MS.

Tutorials (5 hours): Individual work, exercises to be prepared before and during the session.

Case studies (interpretation of MS and MS/MS spectra of biomolecules, interpretation of LC/MS and LC/MS/MS data for reaction monitoring in organic chemistry/biomolecular chemistry).