Protein engineering

Proteins are now widely used as therapeutic tools in human and animal health. Knowledge of peptide and protein synthesis pathways, folding and post-translational modifications is essential before any therapeutic protein biosynthesis can be envisaged. Methods to better characterize these proteins are also essential to guarantee the quality of the proteins produced for therapeutic or industrial use. Protein engineering methods designed to improve their original properties will also be discussed.

The course includes lectures and tutorials by teacher-researchers and researchers.

The aim of this course is to provide students with knowledge of the synthesis and organization of proteins, their study methods and strategies for modifying them for industrial use. The notion of recombinant proteins will then be explored in greater depth in M2.

At the end of the course, students will have acquired skills in :

-Protein structure and folding ;

-Protein analysis methods: proteomics, mass spectrometry, structural analysis, structural bioinformatics;

-Translation and post-translational modifications of proteins ;

-Peptide synthesis ;

-Expression and purification of recombinant proteins;

-Protein engineering and applications (vaccines, industrial enzymes, insulins, de novo proteins).

Students will be able to choose the methods for obtaining and analyzing peptides and proteins best suited to their project. They will be able to implement methods for modifying the structure and/or function of a protein with a view to an industrial application.

Training in biochemistry and cell biology at L3 biology level.

Assessment of knowledge is based on a written final test.