Protein engineering

Proteins are now widely used as therapeutic tools in human and animal health. The knowledge of peptide and protein synthesis pathways, their folding, their possible post-translational modifications is essential before considering any therapeutic protein biosynthesis. Methods to better characterize these proteins are also essential to guarantee the quality of the proteins produced for therapeutic or industrial use. Protein engineering methods to improve their original properties will also be discussed.

The UE includes lectures and tutorials, conducted by teacher-researchers and researchers.

The objective of this course is to provide students with knowledge on the synthesis and organization of proteins, their study methods and strategies to modify them for industrial use. The notion of recombinant proteins will be further developed in M2.

At the end of the training, the student will have acquired skills on :

-Protein structure and folding;

-Protein analysis methods: proteomics, mass spectrometry, structural analysis, structural bioinformatics;

-Translation and post-translational modifications of proteins;

-Peptide synthesis;

-Expression and purification of recombinant proteins;

-Protein engineering and its applications (vaccines, industrial enzymes, insulins, de novo proteins).

The student will be able to choose the methods of obtaining and analyzing peptides and proteins that are best suited to his/her project. He/she will be able to implement methods to modify the structure and/or function of a protein for industrial application.

Training in biochemistry and cell biology at the L3 level in biology.

The control of the knowledge will be realized by a written final control.