Bioprocess Engineering - Batch

Within biotechnologies, bioprocesses correspond to the industrial implementation of living tools (whether enzymes, microorganisms or cells from higher organisms) for the synthesis of products of interest. In this teaching unit (UE), the focus will be on the exploitation of microbial and cellular catalysts. The products of interest can be for example fermented foods (wine, beer, ...), energy molecules (bioethanol, methane, ...), chemical intermediates, or biomedicines (vaccines, monoclonal antibodies, growth factors...). The knowledge, know-how and skills acquired during this course will be transposable to any sector of activity in biotechnology. The examples that will be given will correspond to the outlets targeted by the two training courses (the Agrosciences and Health mentions).

The UE will be dedicated to bioprocesses and to the environment in which the biological reaction will be controlled (the bioreactor). The course will also address the issue of the description and modeling of a biological reaction, including the presentation of the approach applied in bioprocess engineering. The rest of the course will be devoted to the application of this approach to reactors operated in batch mode. The other operating modes will be covered by the M2 course HAV930V "Bioprocess engineering - continuous and fed-batch".

This course includes interactive lectures, tutorials and practical work (practical work in the computer room + personal work in small groups).

Considering the importance of the interaction between biology and engineering sciences in biomanufacturing, this course will allow students to become familiar with the vocabulary used in biomanufacturing and with the parameters that are important at the industrial scale. Thus, students will be able to interact efficiently with the different actors involved in biomanufacturing, engineers or biologists, in R&D or in production, ...

At the end of this course, students will have acquired basic knowledge of bioprocesses (understanding and implementation of a biological reaction, applied approach in bioprocess engineering), bioreactors, different operating modes, choice of operating mode, modeling of a biological reaction.

Students will also have acquired the bioprocess engineering approach (applied in M1 to the BATCH procedure) applicable to any bioprocess with, in particular, the mastery of the following three points

-Exploitation of experimental data (calculation of yields, net velocities, specific velocities...) with the help of usual tools in companies (Excel spreadsheet or free equivalent), interpretation of the results obtained

-Structuring of the data obtained in the form of models

-From the information obtained (from experiments or bibliography), simulation/planning of a culture/production (i.e. dimensioning a simple batch process)

With the proposed practical exercises, students will also be able, at the end of this course, to write an activity report. For this writing, they will have been sensitized on the aspects of communication and capacity of synthesis (choice of the relevant information, choice of the modes of representation of the information in agreement with the message, ...).

Basics of microbiology, metabolism and cell biology (level L3 biology degree or eq.)Basics of differential and integral calculus (level high school: program of mathematics 1st general and technological, applications in common scientific teaching...)

Recommended prerequisites:

The knowledge of the metabolism of catalysts, applied to bioproduction, is essential. This course is therefore closely linked to the course HAV812V "Bioprocess Engineering - Metabolism and Bioproduction" for the M1 Biology-Health / IBIS, and to the course HAA710V "Biological Catalysis and Microbiology" for the M1 Biology-Agrosciences / ICOA.

The control of knowledge is done in continuous integral control (CCI) (personal homework, practical work and final test).