ECTS
5 credits
Component
Faculty of Science
Description
Cell culture is a basic technique in the laboratory and is constantly evolving. It is important to know the basics of cell culture, which are often not well known, even though it is an essential methodology in research and also in industry.
Objectives
The EU has 3 objectives
1-To provide a theoretical basis for cell culture.
2-Give concrete examples of the contribution of cell culture in various fields of research.
3-The recent developments in cell culture (organoids, iPS,...) as well as techniques used in culture (transfection, infection, tracing method,...) will also be discussed
Necessary pre-requisites
No pre-requisites required.
Recommended prerequisites: knowledge in cell biology
Knowledge control
Final test and paper presentation
Syllabus
Course
History of cell culture and its limitations
Culture media and physico-chemical parameters
Equipment, Type of laboratories, RegulationsBioreactor technology (rccs,..)
Cloning and purification techniques
Transfection -Expression in cells
Cellular immortalization
2D and 3D culture model (advantages, limits..)
Organoid model (advantages, limitations,..)
Functional studies on cellular models
Fluorescent Optical Imaging in Cell Culture
Specific cultures: organotypic, explants, organ, co-culture, stem cells, iPS
Reporter genes, fluorescent biomarkers, optogenetics
Measurement of proliferation, apoptosis, metabolism, ... in culture
TD
Oral presentation of scientific articles related to cell culture