Cellular and Molecular Biology 3

This compulsory S4 course allows students to consolidate and deepen the foundations of molecular biology and cell biology acquired in L1.

- Cell biology: The course will cover four major topics: 1) The functioning of the cellular cytoskeleton, 2) Cell adhesion, 3) Protein trafficking, 4) Introduction to cell cycle regulation. Cell biology methodologies will also be presented: immunoprecipitation to highlight protein interactions, fluorescence videomicroscopy to track cell distribution dynamics, and evaluation of the importance of proteins of interest in a cellular process using strategies to modulate their expression (RNA interference, overexpression).

- Molecular biology: After acquiring knowledge about transcription and translation mechanisms in semester 3, we will address gene expression regulation: transcriptional regulation (repressors, activators) and attenuation in prokaryotes, and the basics of expression regulation mechanisms in eukaryotes.

- Knowledge:

Cell biology:

-Understand and assimilate knowledge about the molecular mechanisms involved in the dynamic organization of the cytoskeleton, cell adhesion, vesicular trafficking, and the cell cycle.

-Understanding the basic experimental approaches used in cell biology

Molecular biology: use the knowledge acquired in the previous semester to understand the main principles of gene expression regulation. Understand the experimental approaches used to study these mechanisms.

-Expertise:

Cell biology:

Be able to establish molecular and functional links between the different chapters.

Be able to read and interpret results obtained through classic and varied experimental approaches used in cell biology.

Molecular biology:

Be able to use the knowledge acquired in previous semesters and during biochemistry practicals.

Knowing how to interpret experimental results

-Interpersonal skills:

Understand the various concepts presented and use them to be able to propose hypotheses about how molecular and cellular processes work.

Communicate with teachers and other students to carry out reflection exercises. Be able to formulate a hypothesis based on your knowledge (in cell biology, biochemistry, molecular biology, and physiology) and on experimental results obtained using basic approaches.

Be able to answer questions by justifying your answer clearly and concisely.

Be able to summarize the functioning of a molecular mechanism in the form of a model diagram.

Molecular Biology 2 (HAV306V)