Advanced Cellular and Molecular Biology

This course aims to explore in greater depth, in small groups through tutorials and practical sessions, the fundamental molecular and cellular processes covered in the BMC2 and BMC3 courses, approaching them through more concrete concepts. The lessons will be based on real data (experimental results, scientific articles) to explain the main scientific approaches in simple terms and teach students how to analyze and interpret results (Example 1: showing an in cellulo interaction by expressing labeled proteins in cell lines followed by immunoprecipitation and western blot. Example 2: principle of immunofluorescence, intracellular distribution of an antigen. Example 3: in vitro transcription and translation and interaction study by GST pull-down). Practical work will illustrate some of these basic approaches: cell culture, construction of expression vectors, transfection, immunolabeling, fluorescence microscopy.   

- Knowledge:

- Knowledge and understanding of the fundamental concepts of cell biology and molecular biology

- understand the principles of basic techniques used in cell biology and molecular biology

- Expertise:

- know how to analyze a scientific article figure.

- know how to analyze and interpret the results of a short/simple scientific article and be able to summarize it quickly (level 1 of article analysis).

- know how to maintain a cell line in culture

- know how to perform plasmid transfection on cell lines.

- know how to perform immunocytochemical/fluorescent staining.

- know the basics of using an upright epifluorescence microscope.

- Interpersonal skills:

- work independently and in a group

- Write a report on practical work, following the presentation rules and within the allotted time.

- Comply with the health and safety rules of a practical training room and good laboratory practices related to cell line culture.

- This course allows students to deepen their understanding of the fundamental concepts and techniques of cell biology and molecular biology and is essential for continuing on to the Bachelor's degree program in Molecular and Cell Biology.

-This conceptual and technical deepening course may be of particular interest to students in the Biochemistry and Microbiology L3 program.

- From molecules to cells (S1).

- Biochemistry and molecular biology of the cell (S2).

- Cellular and molecular biology (S3).

- Cell biology and molecular biology (S4)