Analysis of volatile molecules

Knowledge of gas chromatography and mass spectrometry techniques with electron impact ionization and quadrupole mass analyzer for the analysis of volatile organic molecules.

1) GC-MS analysis of volatile organic compounds :

• Electron impact ionization (EI) techniques
• Chemical ionization (CI) techniques
• Quadrupole (Q) analysis techniques
• GC/MS coupling

2) Applications in organic chemistry analysis and characterization of volatile samples.

Hourly volumes* :

CM: 15 H

TD: 5 H

Understand mass spectrometry techniques coupled with gas chromatography (GC-MS) adapted to the analysis of volatile organic molecules.

Be able to interpret GC-MS data.

Understand and analyze the strategy used to characterize a vaporizable sample.

Mass spectrometry, level L3 :

Basic concepts (production/measurement of ions in the gas phase, instrumental diagram)

Final written exam, 2 hours:

• Authorized documents: no
• Non-graphic calculator allowed: yes
• Internet allowed: no

Course: Inductive (problem-based) and deductive pedagogy, Support(s) available on ENT (Moodle): Course documents, TD documents, exam annals and reference publications.

Description of conventional mass spectrometry techniques coupled with gas chromatography for the qualitative analysis of volatile organic molecules.

1. Gas chromatography (3H)

- Principle,

- FID detection,

- Detection by mass spectrometry.

1. Hard ionization methods: (6H)

• Electronic Impact (EI) :

-Production of molecular and fragment ions,

-Description of the main fragmentation families.

• Chemical ionization (CI).

1. Analytical methods: (3H)

• Measuring accuracy and elemental composition,
• Low-resolution quadrupole analyzer.

1. Coupled technique: (3H)

- Principle of GC/MS,

- GC/MS data acquisition.

Tutorials (5 hours): Individual work, exercises to be prepared before and during the session.

Case studies with illustrations in various fields of application (interpretation of EI-MS and GC/EI-MS spectra of volatile organic molecules).