Analysis of volatile molecules

Knowledge of gas chromatography techniques and mass spectrometry with electron impact ionization and quadrupole mass analyzer for the analysis of volatile organic molecules.

1) GC-MS analyses of volatile organic compounds:

• Electron impact (EI) ionization techniques
• Chemical ionization (CI) techniques
• Quadrupole (Q) analysis techniques
• GC/MS Couplings

2) Applications in organic chemistry analysis and characterization of volatile samples.

Hourly volumes*:

CM: 3 p.m.

Tutorial: 5 hours

Understanding gas chromatography-mass spectrometry (GC-MS) techniques suitable for analyzing volatile organic molecules.

Be able to interpret GC-MS data.

Understand and be able to analyze the strategy implemented to characterize a vaporizable sample.

Mass spectrometry, Level 3:

Basic concepts (production/measurement of ions in the gas phase, instrument diagram)

2-hour final written exam:

• Authorized documents: no
• Non-graphing calculator permitted: yes
• Internet access: no

Course: Inductive (problem-based) and deductive teaching methods, Materials available on the digital workspace (Moodle): Course documents, tutorial documents, past exam papers, and reference publications.

Description of conventional mass spectrometry techniques coupled with gas chromatography for the qualitative analysis of volatile organic molecules.

1. Gas chromatography (3 hours)

- Principle,

- FID detection,

- Detection by mass spectrometry.

1. Hard ionization methods: (6H)

• Electronic impact (EI):

-Production of molecular ions and fragment ions,

-Description of the main fragmentation families.

• Chemical ionization (CI).

1. Methods of analysis: (3 hours)

• Measurement accuracy and elemental composition,
• Low-resolution quadrupole analyzer.

1. Coupled technique: (3H)

- Principle of GC/MS,

- Data acquisition in GC/MS.

TD (5 hours): Individual work, exercises to be prepared before and during the session.

Case studies with illustrations in various fields of application (interpretation of EI-MS and GC/EI-MS spectra of volatile organic molecules).