ECTS
5 credits
Training Structure
College of Sciences
Description
This practical course unit is based on mini-projects that apply techniques for the structural analysis of biomolecules (X-ray crystallography, NMR, cryo-EM, mass spectrometry). In addition, students will learn the biophysical and biochemical techniques used to characterize the molecules under analysis and their interactions.
For each approach, students will learn the basic principles, sample preparation requirements, data acquisition, and data analysis.
Objectives
Students will learn how to use the various tools available for the structural characterization of biomolecules and their interactions. In addition, they will learn how to combine these different tools to address a specific biological question.
Mandatory Prerequisites
- Structural Biochemistry: structures and physicochemical properties of amino acids, lipids, and sugars; primary through quaternary structures of proteins; physicochemical bases of molecular interactions.
- Applied Biochemistry: Protein Production and Purification
- Bioinformatics Tools: PyMol, alignments, BLAST, secondary structure prediction
- Physics: fundamentals of light-object interaction, scattering, absorption, diffraction
-Math: complex numbers, Fourier analysis, vector bases, and Euclidean geometry
Recommended prerequisites:
Boot Camp (HAV704V)
Structural Biology (HAV712V)