ECTS
5 credits
Training structure
Faculty of Science
Description
This practical course is based on mini-projects applying structural study techniques for biomolecules (X-ray crystallography, NMR, cryo-EM, mass spectrometry). In addition, students will learn the biophysical and biochemical techniques used to characterize the molecules analyzed and their interactions.
For each approach, students will learn the basic principles, sample preparation requirements, data acquisition, and data analysis.
Objectives
Students will learn how to use the various tools available for the structural characterization of biomolecules and their interactions. In addition, they will learn how to combine these different tools to answer a given biological question.
Mandatory prerequisites
- Structural Biochemistry: structures and physicochemical properties of amino acids, lipids, and sugars; primary to quaternary structure of proteins; physicochemical basis of molecular interactions.
- Technical biochemistry: protein production and purification
- Bioinformatics tools: PyMol, alignments, blast, secondary structure prediction
- Physics: fundamentals of light/object interaction, scattering, absorption, diffraction
-Math: complex numbers, Fourier analysis, vector bases, and Euclidean geometry
Recommended prerequisites:
Bootcamp (HAV704V)
Structural Biology (HAV712V)