Continuous and fed-batch bioprocess engineering

Within biotechnologies, bioprocesses correspond to the industrial implementation of living tools (whether enzymes, microorganisms or eukaryotic cells) for the synthesis of products of interest. This course will focus on the central step of the bioprocess: the biological reaction in a reactor. It will be more particularly devoted to the exploitation of microbial and cellular catalysts. The products of interest can be for example fermented foods (wine, beer, ...), energy molecules (bioethanol, methane, ...), chemical intermediates, or biomedicines (vaccines, monoclonal antibodies, growth factors...). The knowledge and skills acquired during this course will be transposable to any sector of activity. The examples will be given in the fields corresponding to the main outlets of the two courses concerned (Agrosciences and Health). This course is the direct continuation of the M1 course HAV811V "Bioprocess Engineering - Batch". It focuses on the UpStream Processing (USP) aspects of bioprocesses.

The first lectures will allow to resituate the bioprocesses and the approach applied in bioprocess engineering and to briefly recall the Batch mode (pre-requisite of M1). Then, the main part of the course will be devoted to the application of the bioprocess engineering approach to reactors operated in continuous and Fed-Batch mode (or semi-(dis)continuous culture).

Cross-cutting modules will also be offered:

-Transfer management (mixture management, heat transfer, gas transfer) with an important focus on gas transfer and how to ensure the oxygen needs of a culture (kLa, OUR, OTR) -Design of culture media

-Elementary balances (carbon balance and redox balance)

-Development of an indicator for monitoring the biological reaction: the Respiratory Quotient (RQ)

This course includes interactive lectures and tutorials.

An intensive practical application (1 month) carried out in the form of a project is planned for the students of the M2 Biology-Health / IBIS / Bioproduction specialization in the framework of the UEs "Multidisciplinary lab project: from gene to protein". For these students, a strong link is also foreseen with the specialization courses (HAV910V, HAV911V and HAA910V). See the descriptions of these courses for more information.

The main objective of this course is the understanding and implementation of a biological reaction. The objectives already listed in the M1 HAV811V "Bioprocess Engineering -Batch" are still valid and are reinforced.

At the end of this course, thanks to the acquisition of a rational approach in bioprocessing, students will be able to design and dimension a bioprocess (process design), as well as to scale-up a process:

-to critically analyze the information at their disposal,

-to choose the solutions to be implemented in terms of crop management on a pilot or industrial scale according to the objectives and constraints of production

-to precisely define the objectives of the culture

-to carry out a simulation of the culture (to minimize preliminary tests and cultures on a laboratory or pilot scale, to define the operating parameters precisely, to plan the culture in accordance with the human and material means of production, to anticipate the key parameters to be followed for the culture and its control as well as for the implementation of a quality approach...).

In addition, the objective is also to be aware of the challenges related to the implementation in large industrial bioreactors and particularly in terms of transfer (heat transfer, gas transfer).

Basics of microbiology, metabolism and cell biology (L3 level biology degree or eq.)

Basics of differential and integral calculus (high school level: general and technological mathematics program, applications in common scientific education...)

Recommended prerequisites:

Basics of Bioprocess Engineering (M1 level UEs HAV811V "Bioprocess Engineering -BATCH" or eq.)

Applied metabolism in bioproduction (M1 level) UE HAV812V "Bioprocess engineering -

Metabolism and Bioproduction" or HAA710V "Biological Catalysis and Microbiology" or eq.)

The control of knowledge is done in continuous integral control (CCI) (personal homework, group work and final test).