Recombinant protein production engineering

Within biotechnology, the production of recombinant proteins in various prokaryotic and eukaryotic expression systems represents a mature and attractive technological field with high employability. It is also a very important area of research in which many challenges remain to be addressed. The bioproduction of biomedicines (recombinant proteins as well as monoclonal antibodies) represents a major challenge in human therapeutics, but also in many areas of biotechnology (environmental, industrial, agronomic, marine, etc.). Before designing any biological drug involving a bioproduction stage, it is essential to understand the different eukaryotic and prokaryotic expression systems used in biotechnology and to have a comprehensive overview of the landscape and challenges of bioproduction in France, Europe, and around the world.

This course unit includes interactive lectures. It is taught by various academic and industry experts involved in the field.

The objective of this course unit is to provide students with skills in the following areas:

-the market and challenges of bioproduction in Europe and worldwide

-in-depth knowledge of the various prokaryotic and eukaryotic expression systems and their optimization, including:

-protein production in prokaryotic cells (optimization, large-scale production, example of research and development)

-production in yeast cells, particularly Pichia pastoris

-production in baculovirus and insect cell systems

-production in transgenic plants-production in mammalian cells-production and purification of membrane proteins.

The EU program will follow technological developments in the field and is therefore likely to evolve depending on the stakeholders involved.

In-depth knowledge of cellular biology, molecular biology, and biochemistry is required.

Recommended prerequisites:

Additional information:

An intensive one-week practical project directly related to this course unit is planned for students in the M2 Biology-Agroscience / IBION-Tec program and for students in the M2 Biology-Health / IBIS program as part of the course unit "Multidisciplinary lab project 1: from gene to protein, Strain construction & lab scale production" (course unit HAV916V).

Knowledge will be assessed in a final written exam.