Continuous and fed-batch bioprocess engineering

Within biotechnologies, bioprocesses correspond to the industrial implementation of living tools (whether enzymes, microorganisms or eukaryotic cells) for the synthesis of products of interest. This course will focus on the central stage of the bioprocess: the biological reaction in the reactor. In particular, it will focus on the use of microbial and cellular catalysts. The products of interest may be fermented foods (wine, beer, etc.), energy molecules (bioethanol, methane, etc.), chemical intermediates or biomedicines (vaccines, monoclonal antibodies, growth factors, etc.). The knowledge and skills acquired in this course can be applied to any sector of activity. Examples will be given in fields corresponding to the main outlets of the two courses concerned (Agrosciences and Health). This course is a direct continuation of the M1 course HAV811V "Bioprocess Engineering -Batch". It focuses on the UpStream Processing (USP) aspects of bioprocessing.

The first lectures will provide an overview of bioprocesses and the approach applied in bioprocess engineering, as well as a brief reminder of the Batch mode (M1 prerequisite). The bulk of the course will then be devoted to applying the bioprocess engineering approach to reactors operating in continuous and Fed-Batch (or semi-(dis)continuous culture) modes.

Cross-disciplinary modules will also be offered:

-Transfer management (mixture management, heat transfer, gas transfer) with a focus on gas transfer and how to ensure a culture's oxygen requirements (kLa, OUR, OTR) -Design of culture media

-Elementary balances (carbon and redox balances)

-Development of a biological reaction monitoring indicator: the Respiratory Quotient (RQ)

This course includes interactive lectures and tutorials.

Students in the M2 Biology-Health / IBIS / Biomanufacturing specialization will be able to put their project into practice intensively (1 month) as part of the "Multidisciplinary lab project: from gene to protein" UEs. For these students, a strong link is also provided with the specialization courses (HAV910V, HAV911V and HAA910V). Please refer to the descriptions of these UEs for further information.

The main objective of this course is to understand and implement a biological reaction. The objectives already listed in the M1 HAV811V "Bioprocess Engineering -Batch" are still valid and are reinforced.

At the end of this course, thanks to the acquisition of a rational approach to bioprocessing, students will be able to design and dimension a bioprocess (process design), as well as scale-up a process:

-critically analyze the information available to them,

-choose the solutions to be implemented in terms of crop management on a pilot or industrial scale, depending on production objectives and constraints

-precisely define cultivation objectives

-simulate cultivation (to minimize preliminary trials and cultivation at lab or pilot scale, to define operating parameters precisely, to plan cultivation in line with human and material production resources, to anticipate the key parameters to be monitored for cultivation and its control, and to implement a quality approach...).

In addition, the aim is also to be aware of the challenges associated with implementation in large-scale industrial bioreactors, particularly in terms of transfer (heat transfer, gas transfer).

Basic knowledge of microbiology, metabolism and cell biology (L3 biology degree or equivalent)

Basics of differential and integral calculus (high school level: 1st year general and technological mathematics syllabus, applications in common science teaching...).

Recommended prerequisites:

Bioprocess engineering basics (M1 level UEs HAV811V "Bioprocess engineering -BATCH" or eq.)

Applied metabolism in bioproduction (M1 level UE HAV812V "Bioprocess engineering -

Metabolism and bioproduction" or HAA710V "Biological catalysis and microbiology" or eq.)

Assessment of knowledge is based on continuous assessment (CCI) (personal assignments, group work and final test).