Cell culture

Cell culture is a basic technique in the laboratory and is constantly evolving. It is important to know the basics of cell culture, which are often not well known, even though it is an essential methodology in research and also in industry.

The EU has 3 objectives

1-To provide a theoretical basis for cell culture.

2-Give concrete examples of the contribution of cell culture in various fields of research.

3-The recent developments in cell culture (organoids, iPS,...) as well as techniques used in culture (transfection, infection, tracing method,...) will also be discussed

No pre-requisites required.

Recommended prerequisites: knowledge in cell biology

Final test and paper presentation

Course

History of cell culture and its limitations

Culture media and physico-chemical parameters

Equipment, Type of laboratories, RegulationsBioreactor technology (rccs,..)

Cloning and purification techniques

Transfection -Expression in cells

Cellular immortalization

2D and 3D culture model (advantages, limits..)

Organoid model (advantages, limitations,..)

Functional studies on cellular models

Fluorescent Optical Imaging in Cell Culture

Specific cultures: organotypic, explants, organ, co-culture, stem cells, iPS

Reporter genes, fluorescent biomarkers, optogenetics

Measurement of proliferation, apoptosis, metabolism, ... in culture

TD

Oral presentation of scientific articles related to cell culture