Cell culture

Cell culture is a basic laboratory technique that is constantly evolving. It is important to know the basics, which are often poorly understood, even though it is an essential methodology in research and industry.

The EU has 3 objectives

1-Theoretical basis of cell culture.

2-Give concrete examples of the contribution of cell culture in various fields of research.

3-Recent developments in cell culture (organoids, iPS, etc.) and techniques used in culture (transfection, infection, tracing methods, etc.) will also be discussed.

No prerequisites required.

Recommended prerequisites: knowledge of cell biology

Final test and article presentation

Courses

History of cell culture and its limitations

Culture media and physico-chemical parameters

Equipment, Laboratory types, RegulationsBioreactor technology (rccs,..)

Cloning and purification techniques

Transfection -Expression in cells

Cell immortalization

2D and 3D culture models (advantages, limitations, etc.)

Organoid model (advantages, limitations, etc.)

Functional studies on cell models

Fluo Optical Imaging in Cell Culture

Specific cultures: organotypic, explants, organ, co-culture, stem cells, iPS

Reporter genes, fluorescent biomarkers, optogenetics

Measurement of proliferation, apoptosis, metabolism, etc. in culture

TD

Oral presentation of scientific articles related to cell culture