Advanced Cell Biology 2: Key Concepts

This course is designed to complete the notions of cell biology seen in L2 in BCM2 and BCM3 through the study of physiological and pathological situations such as cancers.

These courses will introduce students to the importance of finding a study model (cell lines or organisms for in vivo study (Drosophila, C. elegans, Zebrafish) adapted to answer a question and highlight a molecular mechanism.

The lectures will allow the manipulation of advanced concepts of cell biology in connection with the knowledge of the most commonly used methodologies in cell biology such as flow cytometry, advanced techniques of fluorescence and electron microscopy, proteomics as well as the knowledge of experimental models such as cells in culture, genetically modified or not animal models (C. elegans, zebrafish, transgenic mice, KO, KI...)

- Knowledge:

- knowledge and understanding of fundamental concepts of cell biology and molecular biology in relation to normal and pathological physiological mechanisms

- understand the principles of advanced techniques used in cell biology and molecular biology

- Knowledge of experimental models (cells in culture, droso, xenopes, c elegans, mouse, zebrafish, ...)

- Knowledge of the molecular mechanisms involved in RNA interference approaches, obtaining cells from KO mice, expression of dominant negative or constitutively active mutants

- Basic knowledge of proteomics, photonic and electron microscopy approaches and their application in cell biology.

- Know-how :

- know how to make the link between a deregulation of a cellular mechanism and a pathological disorder

- To be able to integrate all of one's knowledge in cell biology, physiology, molecular biology, biochemistry, genetics, to answer questions concerning cellular mechanisms/functions.

- know how to choose the most appropriate cell biology technique to answer a specific scientific question propose solutions to improve an experimental approach or propose complementary parallel approaches

- Know how to be :

- work independently and in groups

- From molecules to cells (S1).

- Biochemistry and molecular biology of the cell (S2).

- Cellular and molecular biology (S3).

- Cellular and molecular biology (S4)

- This UE allows a deepening of the fundamental concepts and techniques of Cellular and Molecular Biology and is essential to follow the L3 Molecular and Cellular Biology course.

-This conceptual and technical deepening UE can be interesting in particular for the L3 Biochemistry course.