Advanced cellular and molecular biology

The aim of this course is to take a more in-depth look at the fundamental molecular and cellular processes seen in BMC2 and BMC3, using more concrete concepts in small groups. Lessons will be based on real data (experimental results, scientific articles) to explain the main scientific approaches in simple terms, and to learn how to analyze and interpret results (Example 1: show an interaction in cellulo by expression of tagged proteins in cell lines followed by immunoprecipitation and western-blot. Example 2: principle of immunofluorescence, intracellular distribution of an antigen. Example 3: in vitro transcription and translation and GST-pull down interaction studies.) Practical exercises will illustrate some of these basic approaches: cell culture, expression vector construction, transfection, immunolabeling, fluorescence microscopy.   

- Knowledge :

- knowledge and understanding of the fundamental concepts of cell biology and molecular biology

- understand the principles of the basic techniques used in cell biology and molecular biology

- Know-how :

- know how to analyze a figure in a scientific article.

- know how to analyze and interpret the results of a short/simple scientific article and be able to produce a rapid synthesis (level 1 article analysis).

- how to maintain a cell line in culture

- perform plasmid transfection on cell lines.

- know how to perform immunocytochemical/fluorescent labeling.

- know the basics of using an epifluorescence upright microscope.

- People skills :

- work independently and in groups

- Write a report on practical work, respecting the rules of presentation and the time allowed.

- respect the hygiene and safety rules of a laboratory and the good laboratory practices related to cell line culture

- This course provides an in-depth understanding of the fundamental concepts and techniques of Cellular and Molecular Biology, and is essential for the L3 Molecular and Cellular Biology pathway.

-This advanced conceptual and technical course may be of particular interest to L3 Biochemistry and Microbiology students.

- From molecules to cells (S1).

- Biochemistry and molecular biology of the cell (S2).

- Cellular and molecular biology (S3).

- Cellular and molecular biology (S4)