Practical approach to bacterial biodiversity

This practical course aims to apply the students' knowledge of microbiology and molecular biology to the identification of environmental bacteria.

The quantitative and qualitative analysis of the bacterial population present in a soil sample will be done classically by identifying the species by the means of classical bacteriology in successive steps: 1) isolation of the bacterial flora; 2) diagnosis of family and genus using conventional media and tests; 3) diagnosis of the species using API System galleries

Molecular biology techniques now allow the identification of bacteria present in a sample without the need to culture them. This approach requires access to a sequencing platform and will also be carried out during the course of the project, which will allow the two approaches to be compared. The sequencing results obtained will allow a bioinformatics analysis of the rrsA gene specifying the RNA16S of the isolated bacteria.

- Practical know-how in microbiology and molecular biology

- Use your knowledge and develop your ability to analyze results

- Develop a critical and synthetic mind

- Mobilize simple bioinformatics tools

basic knowledge of bacteriology and molecular biology