Recombinant protein expression systems

In the field of health biotechnology, recombinant proteins can be produced in different cell types, known as expression systems, which can be prokaryotic or eukaryotic. This is a mature and attractive technological field with high employability. Indeed, the bioproduction of biomedicines (proteins, monoclonal antibodies) represents a major therapeutic challenge in humans. Before designing any biological drug involving a bioproduction step, it is essential to understand the different eukaryotic and prokaryotic expression systems used in biotechnology. 

This course unit includes interactive lectures, presentations by professionals, and practical work during which the knowledge acquired in class will be put into practice: cell culture, production clone controls, recombinant protein production in eukaryotic systems, and production controls. 

Skills targeted by the EU: 

- Understand the expression systems used in industry: uses and advantages, main strains used, methods for obtaining producer clones, limitations 

-Practical skills: 

-Prepare and conduct eukaryotic cell culture at the laboratory scale (adherent and suspension cells, preparation of a cell bank, preparation of media) 

-Characterization of the producer clone (viability, sterility, stability) 

Cellular and molecular biology, microbiology 

Continuous assessment of the theoretical part 

Continuous assessment of the practical component, including the writing of a practical work report.