Recombinant protein expression systems

In the field of healthcare biotechnology, recombinant proteins can be produced in different cell types, called expression systems, which can be prokaryotic or eukaryotic. This is a mature and attractive technological field with high employability potential. The bioproduction of biomedicines (proteins, monoclonal antibodies) represents a major therapeutic challenge for human beings. Before designing any biological drug involving a biomanufacturing stage, it is essential to be familiar with the different eukaryotic and prokaryotic expression systems used in biotechnology. 

This course combines interactive lectures, presentations by professionals and practical work in which the knowledge acquired in class is put into practice: cell culture, control of the producing clone, recombinant protein production in eukaryotic systems, production controls. 

Skills targeted by the EU : 

- Know the expression systems used in the industry: uses and advantages, main strains used, methods for obtaining producer clones, limitations. 

-Practical skills : 

Prepare and run laboratory-scale eukaryotic cell culture (adherent and suspension cells, cell bank preparation, media preparation). 

-Characterization of the producing clone (viability, sterility, stability) 

Cellular and molecular biology, microbiology 

Integral continuous assessment of the theoretical part 

Continuous assessment of the practical part, including a practical report.