Molecular engineering and construction of producer clones

The main aim of this course is to enable students to acquire theoretical and practical knowledge in molecular engineering. This means being able to integrate a gene coding for a molecule of interest and express it in an adapted organism. This is the preparation stage of biomanufacturing.   

In order to produce a molecule of interest, it is necessary to understand upstream the mechanisms and molecular tools at the origin of a modified organism (prokaryotic and/or eukaryotic). Knowledge of the enzymes involved in genetic modification and the verification/validation of this modification via vectorization will be covered. Extraction, purification and expression methods will be covered in practical exercises. In addition, a comprehensive overview of the benefits of molecular engineering in healthcare will be presented.

Skills targeted by the EU : 

- Understanding the molecular mechanisms of genetic enzymes// involved in genetic modification  

- Know the most common PCR techniques and methods 

- Knowing the different ways to vectorize a gene 

- Master the techniques and tools of molecular cloning and verification 

- Carry out a scientific protocol independently 

- Validate, interpret and present results  

- Know the limits (advantages and disadvantages) of molecular biology tools. 

L2 Life Sciences, L2 Health Engineering, BUT Biological Engineering, BTS Biotechnologies, BTSA Biological, Biotechnological, Agricultural and Environmental Analysis (ANABIOTEC) and equivalent courses. 

Continuous written assessment of theoretical content, including a final exam to validate all knowledge. Assessment of practical work on handling and reporting results.