Immunotechnologies

Immunotechnology is a field of biotechnology which uses knowledge of fundamental immunology to develop technological methods for therapeutic, diagnostic or analytical purposes. This course will show how immunotechnology can be used at different stages in the biomanufacturing of a biomedicine product, from research and development to quality control. 

The main objectives of this UE are to :  

-Recall basic knowledge of fundamental immunology in order to understand the structure of antibodies and their functions, as well as the immunogenicity of biomedicines. 

-Present the biotechnological tools used to generate and select monoclonal and polyclonal antibodies  

-Present analytical methods for characterizing antibodies (identity, function)  

This course will cover a number of theoretical themes: 

The immune system 

The antigen-antibody reaction 

Epitopic analysis 

Antibody engineering  

Analytical methods  

This course includes lectures, a practical session and practical work to apply the immunotechniques taught in this course.

Fundamental immunology and cell biology

Knowledge is tested by means of a comprehensive continuous assessment (CCI), which includes the grade for the theoretical part of the test and the grade for the practical part.  

Skills targeted by the EU : 

- Knowledge of the principle of each method used for therapeutic antibody analysis  

- Knowledge of monoclonal antibodies (format, mechanism, therapeutic application) and the various production strategies.  

- Set up and optimize experimental protocols (ELISA, Western blot, immunofluorescence, flow cytometry) 

- Interpretation of analytical results from an analytical method