Advanced Cell Biology 2: Key concepts

This course is designed to complement the notions of cell biology covered in L2 in BCM2 and BCM3 through the study of physiological and pathological situations such as cancer.

These lessons will introduce students to the importance of finding a suitable study model (cell lines or organisms for in vivo study (Drosophila, C. elegans, Zebrafish) to answer a question and highlight a molecular mechanism.

TDs will allow the manipulation of advanced concepts of cell biology in connection with knowledge of the most commonly used methodologies in cell biology such as flow cytometry, advanced techniques of fluorescence and electron microscopy, proteomics as well as knowledge of experimental models such as cells in culture, genetically modified or non-genetically modified animal models (C. elegans, zebrafish, transgenic mice, KO, KI...).

- Knowledge :

- knowledge and understanding of the fundamental concepts of cell biology and molecular biology in relation to normal and pathological physiological mechanisms

- understand the principles of advanced techniques used in cell biology and molecular biology

- Knowledge of experimental models (cell cultures, droso, xenopes, c elegans, mice, zebrafish, etc.)

- Knowledge of the molecular mechanisms involved in RNA interference approaches, obtaining cells from KO mice, expression of dominant-negative or constitutively active mutants.

- Basic knowledge of proteomics, photonic and electron microscopy approaches and their field of application in cell biology.

- Know-how :

- make the link between a deregulation of a cellular mechanism and a pathological disorder

- Integrate knowledge of cell biology, physiology, molecular biology, biochemistry and genetics to answer questions concerning cellular mechanisms/functions.

- choose the most appropriate cell biology technique to answer a specific scientific question suggest solutions to improve an experimental procedure or propose complementary parallel approaches

- People skills :

- work independently and in groups

- From molecules to cells (S1).

- Biochemistry and molecular biology of the cell (S2).

- Cellular and molecular biology (S3).

- Cellular and molecular biology (S4)

- This course provides an in-depth understanding of the fundamental concepts and techniques of Cellular and Molecular Biology, and is essential for the L3 Molecular and Cellular Biology pathway.

-This advanced conceptual and technical course could be particularly interesting for the L3 Biochemistry pathway.