Structural Biochemistry

This course offers an in-depth look at the structural biochemistry of biomolecules, with a particular focus on proteins and nucleic acids.

The basic concepts and nomenclature used to analyze 3D protein structures are briefly reviewed (Ramachandran diagram, structural motif and domain, folding, family, superfamily, etc......). These notions are complemented by a study of the stability and dynamics of biomolecules.

The structural classification of proteins is detailed according to the 4 main types of folding. Structure-function relationships are illustrated using examples of proteins. Specificities of membrane protein structures (integral proteins, membrane-bound proteins) are discussed.

The main tools for modeling and predicting secondary and tertiary structures are presented.

The different structures and functions of nucleic acids are studied. Protein-nucleic acid complexes are described from a structural point of view (main recognition motifs, ...) and the notions of recognition specificity are detailed.

This course is illustrated by tutorials. These involve familiarizing students with the main databases used in structural biology, as well as with PyMol software for 3D structure analysis.

This course provides an understanding of biological issues at the molecular level. Students will be able to describe and analyze all biomolecule structures, identify the molecular determinants of biomolecule interactions and understand their importance. They will be able to explore structure-function relationships.

On completion of this course, students should be able to study all types of biomolecules in 3D using the software of their choice. They should be able to give precise structural information, such as the distance between a protein and a ligand, as well as the spatial organization.

HAV201V, HAV204V