Practical work in biochemistry

This course enables students to consolidate and deepen their practical management of numerous experimental data obtained over a week of practical work during a blocked period (5 consecutive days). These data are obtained following the elaboration of many different protocols, keeping in mind to ensure the best reproducibility of the preparations carried out and to have the best speed of execution in the preparation, realization and analysis of the different experiments. A high degree of autonomy in setting up protocols will be encouraged, leading ultimately to experimental mastery and autonomy. This practical work will also enable students to work in groups (in pairs or trios, depending on capacity and number of students), and to write a report recording the protocols carried out, all the experimental data obtained and their analysis, in order to determine a wide range of biochemical parameters. A significant part of the assessment will be based on the students' ability to generate, manage, exploit and analyze raw experimental data with the utmost rigor.

- Knowledge :

-Quickly set up and design a reliable experimental protocol.

Master the methods of reagent preparation and experiment set-up to guarantee maximum experimental reproducibility.

-Analyze, exploit and maintain an objective view of the relevance of raw data obtained.

-Know how to associate the results obtained with possible practical failures in order to improve subsequent experiments.

-Gain in experimental autonomy.

-Write clear, precise, rigorous, analytical and critical experimental reports.

-Know-how :

-Understand the experimental and practical constraints involved in obtaining a raw data set that needs to be processed over a relatively long period (several consecutive days).

-Attaching the utmost rigor to the production and use of a wide range of data.

-Take an objective, critical look at your results and conclusions.

-Teamwork skills.

-People skills :

-Independently design a clear, rigorous experimental protocol.

-Manage a wide range of experimental results with an objective commitment.