Practical Work in Biochemistry

This course unit allows students to consolidate and deepen their practical management of the large amount of experimental data obtained during a week of practical work in a block period (5 consecutive days). This data is obtained following the development of numerous different protocols, with a view to ensuring the best possible reproducibility of the preparations carried out and the fastest possible execution in the preparation, implementation, and analysis of the various experiments. A high degree of autonomy in the implementation of protocols will be encouraged, ultimately leading to experimental mastery and autonomy. These practical sessions also allow for group work (in pairs or threes, depending on capacity and numbers) and the writing of a report detailing the protocols carried out, all the experimental data obtained, and their analysis in order to determine a wide range of biochemical parameters. A significant part of the assessment will be based on the students' ability to generate, manage, exploit, and analyze raw experimental data with the utmost rigor.

- Knowledge:

-Know how to quickly set up and develop a reliable experimental protocol.

-Mastering reagent preparation methods and setting up experiments to ensure the best experimental reproducibility.

-Analyze, utilize, and maintain an objective view of the relevance of the raw data obtained.

-Know how to associate results obtained with possible practical failures in order to improve subsequent experiments in a relevant manner.

-Gain experimental autonomy.

-Know how to write a clear, precise, rigorous, analytical, and critical experimental report.

 -Expertise:

-Understanding the experimental and practical constraints involved in obtaining a set of raw data that must be analyzed over a relatively long period (several consecutive days).

-Apply the utmost rigor to the creation and use of a diverse and varied set of data.

-Provide an objective and critical perspective on your results and conclusions.

-Ability to work in a team.

 -Interpersonal skills:

-Know how to independently develop a clear and rigorous experimental protocol.

-Know how to manage a wide range of experimental results by engaging with them objectively.