Advanced Cell Biology 2: Key Concepts

This course unit aims, in both lecture and tutorial formats, to supplement the concepts of cell biology covered in L2 in BCM2 and BCM3 through the study of physiological and pathological conditions such as cancer.

These courses will introduce students to the importance of finding a suitable model system (cell lines or organisms for in vivo studies (Drosophila, C. elegans, zebrafish)) for answering a question and demonstrating a molecular mechanism.

The tutorials will enable students to work with advanced concepts in cell biology in conjunction with knowledge of the most commonly used methodologies in cell biology, such as flow cytometry, advanced fluorescence microscopy and electron microscopy techniques, proteomics, and knowledge of experimental models such as cultured cells, genetically modified and non-genetically modified animal models (C. elegans, zebrafish, transgenic mice, KO, KI, etc.). zebrafish, transgenic mice, KO, KI, etc.).

- Knowledge:

- Knowledge and understanding of the fundamental concepts of cell biology and molecular biology in relation to normal and pathological physiological mechanisms.

- understand the principles of advanced techniques used in cell biology and molecular biology

- Knowledge of experimental models (cell cultures, Drosophila, Xenopus, C. elegans, mice, zebrafish, etc.)

- Knowledge of the molecular mechanisms involved in RNA interference approaches, obtaining cells from KO mice, expression of dominant negative or constitutively active mutants

- Basic knowledge of proteomics, photonic and electron microscopy approaches and their field of application in cell biology.

- Expertise:

- know how to link the deregulation of a cellular mechanism to a pathological disorder

- Be able to integrate all of one's knowledge of cell biology, physiology, molecular biology, biochemistry, and genetics to answer questions about cellular mechanisms/functions.

- Know how to choose the most appropriate cell biology technique to answer a specific scientific question Propose solutions to improve an experimental approach or propose complementary parallel approaches

- Interpersonal skills:

- work independently and in a group

- From molecules to cells (S1).

- Biochemistry and molecular biology of the cell (S2).

- Cellular and molecular biology (S3).

- Cell biology and molecular biology (S4)

- This course allows students to deepen their understanding of the fundamental concepts and techniques of cell biology and molecular biology and is essential for continuing on to the Bachelor's degree program in Molecular and Cell Biology.

-This conceptual and technical deepening course may be of particular interest to students in the L3 Biochemistry program.