Biological investigations

This EU is dedicated to biological markers. It is a preliminary introduction to detection and diagnostic techniques. It covers various aspects of biomarking:

Molecular markers/techniques for identification through genomic analysis in medicine and agronomy.

1) Concept of polymorphism and detection technique: RFLP/ER nucleic acid probes

2) RFLP markers and other genetic markers: SNP, STR.

3) Search for new molecular markers: differential screening of cDNA libraries / subtractive libraries / Transcriptomics

4) Other genomic analyses of polymorphism: AFLP / DNA fingerprinting.

Identification techniques in the food industry using immunological techniques

1) Basic concepts in immunological techniques
2) Agglutination reactions
3) Immunoenzymatic assay methods
Case studies of applications in the agri-food industry:
- study of the beet rhizomania diagnostic kit (sandwich ELISA)
- determination of ochratoxin A in cereals (competitive ELISA)
- assessment of fish freshness by histamine determination (competitive ELISA)

Biochemical identification of protein markers and others (metabolites)

1) Fundamentals of chromatography and physical characterization of a spectrum (the interactions involved in each case and the solvents used to implement them).

2) Affinity chromatography

2.1) Principle of this type of analysis

2.2) Search for the best tag for the preparation of a specific gel. 

2.3) Their usefulness for different fields of research investigation.

3) Study of protein-protein, protein-DNA, and other interactions...

4) HPLC, FPLC, and gas chromatography.

- Mastery of molecular markers for molecular analysis and diagnosis

- Proficiency in antigen markers and common immunological analysis techniques.

- Proficiency in chromatographic techniques for analysis and fractionation.