Cellular and Molecular Biology 3

This compulsory UE in S4 enables students to consolidate and deepen the foundations of molecular and cell biology acquired in L1.

- Cell biology: 4 major themes will be covered: 1) Cell cytoskeleton function, 2) Cell adhesion, 3) Protein trafficking, 4) Introduction to cell cycle regulation. Cell biology methodologies will also be presented: immunoprecipitation to highlight protein interactions, fluorescence videomicroscopy to monitor cell distribution dynamics, assessment of the importance of proteins of interest in a cellular process by strategies to modulate their expression (RNA interference, overexpression).

- Molecular biology : After acquiring knowledge of transcription and translation mechanisms in Semester 3, we'll move on to the regulation of gene expression: transcriptional regulation (repressor, activator) and attenuation in prokaryotes, the basics of expression regulation mechanisms in eukaryotes.

- Knowledge :

Cell Biology :

-Understand and assimilate knowledge of the molecular mechanisms involved in the dynamic organization of the cytoskeleton, cell adhesion, vesicular trafficking and the cell cycle.

-Understand the basic experimental approaches used in cell biology

Molecular biology: use the knowledge acquired in the previous semester to understand the main principles of gene expression regulation. Understand the experimental approaches used to study these mechanisms.

-Know-how :

Cell Biology :

Be able to establish molecular and functional links between the various chapters.

Be able to read and interpret results obtained using a wide range of classical experimental approaches used in cell biology.

Molecular biology :

Be able to use the knowledge acquired in previous semesters and during Biochemistry practical work.

Interpret experimental results

-People skills :

Understand the various concepts presented and use them to propose hypotheses on the functioning of molecular and cellular processes.

Communicate with teachers and other students to carry out brainstorming exercises. Be able to formulate a hypothesis based on knowledge (in cell biology, biochemistry, molecular biology and physiology) and on experimental results obtained using basic approaches.

Be able to answer questions and justify answers clearly and concisely.

Be able to synthesize the functioning of a molecular mechanism in the form of a model diagram.

Molecular Biology 2 (HAV306V)