Biochemistry, 3rd Semester

This required course enables students to consolidate the fundamentals of biochemistry acquired in the first year of undergraduate studies by approaching the discipline through a cross-disciplinary study of enzymes involved in cellular metabolism, particularly glycolysis. Several areas of biochemistry will be covered: the fundamentals of Michaelis-Menten enzyme kinetics and a description of the metabolic reactions involved in glycolysis. Finally, the technical aspects will be addressed through the presentation and analysis of techniques used to measure enzyme activity and to purify, quantify, and detect proteins.

Knowledge:

Understand the basic principles and concepts of Michaelis-Menten kinetics and cellular energetics in order to understand the regulation of metabolism.

Basic knowledge of the structure and metabolism of carbohydrates, particularly glycolysis.

Understand that the “spontaneity” of the reactions that make up a metabolic pathway in cells is linked to a combination of intracellular factors (variable concentrations of different metabolites over time) and extracellular factors (concentrations of secreted metabolites, hormonal signals, etc.).

-Expertise:

Locate and quantify enzymatic activity; measure a reaction rate. Quantify a metabolite.

Understand and apply common spectrophotometric assay and analysis techniques; master common separation and purification methods.

Understanding intermolecular interactions.

-Interpersonal skills:

Work in pairs,

Using the workspace (workbenches and shared workspaces),

Communicate with teachers,

Adhere to the specified work hours,

Submit work by the deadline.

From Molecules to Cells (Bachelor's), Biochemistry and Cell Biology (Bachelor's)