Biochemistry S3

This compulsory course allows students to consolidate the fundamentals of biochemistry acquired in the first year by approaching this discipline through a cross-disciplinary study of enzymes involved in cellular metabolism, particularly glycolysis. Several areas of biochemistry will be covered: the fundamentals of Michaelian enzymology and a description of the metabolic reactions involved in glycolysis. Finally, the technical aspect will be addressed through the presentation and analysis of techniques for measuring enzyme activity and purifying, quantifying, and detecting proteins.

Knowledge:

Know the basic notions and concepts of Michaelian enzymology and cellular energetics in order to understand metabolic regulation.

Basic knowledge of the structure and metabolism of carbohydrates, particularly glycolysis.

Understanding that the "spontaneity" of reactions constituting a metabolic pathway in cells is linked to a set of intracellular factors (variable concentrations of different metabolites over time) and extracellular factors (concentration of secreted metabolites, hormonal signals, etc.).

-Expertise:

Locate and measure enzyme activity, measure reaction speed. Measure a metabolite.

Understand and use common spectrophotometric dosing and analysis techniques, master common separation and purification methods.

Understanding intermolecular interactions.

-Interpersonal skills:

Work in pairs,

Use the workspace (workbenches and shared workspaces),

Communicate with teachers,

Respect the given working time,

Submit work on time.

From Molecules to Cells (L1), Biochemistry and Cell Biology (L1)