Cell culture

Cell culture is a basic technique in laboratories and is constantly evolving. It is important to understand its fundamentals, which are often poorly understood despite being an essential methodology in research and industry.

The EU has three objectives

1-Provide theoretical foundations of cell culture.

2-Provide concrete examples of the contribution of cell culture in various fields of research.

3-Recent developments in cell culture (organoids, iPS, etc.) will also be discussed, as well as techniques used in culture (transfection, infection, tracing methods, etc.).

No prerequisites necessary.

Recommended prerequisites: knowledge of cell biology

Final exam and article presentation

Class

History of cell culture and its limitations

Culture media and physicochemical parameters

Equipment, Types of laboratories, Regulations, Bioreactor technology (RCCS, etc.)

Cloning and purification techniques

Transfection - Expression in cells

Cell immortalization

2D and 3D Culture Model (advantages, limitations, etc.)

Organoid model (advantages, limitations, etc.)

Functional studies on cell models

Fluorescent Optical Imaging in Cell Culture

Specific cultures: organotypic, explants, organ, co-culture, stem cells, iPS

Reporter genes, fluorescent biomarkers, optogenetics

Measurement of proliferation, apoptosis, metabolism, etc. in culture

TD

Oral presentation of scientific articles related to cell culture