Cell Culture

Cell culture is a fundamental technique in laboratories and is constantly evolving. It is important to understand its basics, which are often poorly understood, even though it is an essential methodology not only in research but also in industry.

The EU has three objectives

1. Provide a theoretical foundation in cell culture.

2-Provide concrete examples of the contributions of cell culture to various fields of research.

3-Recent developments in cell culture (organoids, iPS cells, etc.) will also be discussed, as well as techniques used in cell culture (transfection, infection, tracing methods, etc.)

No prerequisites are required.

Recommended prerequisites: knowledge of cell biology

Final Exam and Article Presentation

Classes

The History of Cell Culture and Its Limitations

Culture media and physicochemical parameters

Equipment, Types of Laboratories, Regulations, Bioreactor Technology (RCCS, etc.)

Cloning and Purification Techniques

Transfection—Expression in Cells

Cell immortalization

2D and 3D Culture Models (Advantages, Limitations, etc.)

Organoid model (advantages, limitations, etc.)

Functional studies using cellular models

Fluorescence Optical Imaging in Cell Culture

Specialized cultures: organotypic cultures, explants, organ cultures, co-cultures, stem cells, iPS cells

Reporter genes, fluorescent biomarkers, optogenetics

Measurement of proliferation, apoptosis, metabolism, etc., in cell culture

TD

Oral presentations of scientific articles related to cell culture