ECTS
3 credits
Component
Faculty of Science
Description
Within biotechnology, the production of recombinant proteins in different prokaryotic and eukaryotic expression systems represents a mature and attractive technological field with a high employability. It is also a very important field of research in which many challenges remain. The bioproduction of biomedicines (recombinant proteins but also monoclonal antibodies) represents a major challenge in human therapeutics, but also in many fields of biotechnology (environmental, industrial, agronomic, marine...). Before designing any biological drug involving a biomanufacturing step, it is essential to know the different eukaryotic and prokaryotic expression systems used in biotechnologies as well as to have a complete overview of the biomanufacturing panorama and stakes in France, in Europe and in the world.
This UE includes interactive lectures. It is provided by various academic and industrial speakers involved in the field.
Objectives
The objective of this course is to provide students with skills in the following areas
-the market and the challenges of bioproduction in europe and in the world
In-depth knowledge of the various prokaryotic and eukaryotic expression systems and their optimization, including
Protein production in prokaryotic cells (optimization, large-scale production, example of research and development)
-the production of yeast cells, especially Pichia pastoris
-production in baculovirus and insect cell systems
-production in transgenic plants-production in mammalian cells-production and purification of membrane proteins.
The EU program will follow the technological developments in the field and will therefore be likely to evolve according to the speakers mobilized.
Necessary pre-requisites
Extensive knowledge of cell and molecular biology and biochemistry is required.
Recommended prerequisites:
Additional information:
An intensive one-week practical application carried out in the form of a project directly related to this UE is planned for the students of the M2 Biology-Agroscience / IBION-Tec and for the students of the M2 Biology-Health / IBIS in the framework of the UEs "Multidisciplinary lab project 1: from gene to protein, Strain construction & lab scale production" (UE HAV916V).
Knowledge control
A final written exam will be held to verify the knowledge of the student.