Biotechnology for plant breeding

This module begins with an upgrade in molecular and cellular biology. Then, the courses present the impact of biotechnologies on genetic progress. Finally, a description of the different biotechnologies useful in plant breeding is carried out. These biotechnologies are classified into two groups: (i) those that make it possible to create or propagate interesting mutations (mutagenesis, polyploidization, haplo-diploid methods, in vitro culture, transgenesis, genome editing, etc.) and (ii) those that promote the recombination of mutations (interspecific crosses, somatic hybridization, molecular labeling, etc.). These courses are illustrated by interventions carried out by professionals.

Examples of courses taught by professionals:

• Frédéric BAKRY (CIRAD) Banana biotechnology
• Léo HERBERT (CIRAD): CrispR on vines and rice
• Sébastien TISNE (CIRAD): biotechnologies in oil palm improvement

9 hours of practical work on in vitro cell cultures (tobacco, potato, carrot cell culture,...) and microsatellite genotyping and QTL research on a biparental rice population.

Knowledge

• Acquire the basics of biotechnologies that a technician in a seed company or as an assistant breeder may have to deal with.
• Acquire a general knowledge of plant biotechnologies.

Know-how

• Know how to handle under aseptic conditions.
• A polymorphism search for microsatellite markers
• Perform a QTL search.

Hourly volumes :

            CM: 18

            TD : 9

            TP: 9

none

Assessment is based on continuous assessment, including a presentation note, a TP report note and a final exam.