A practical approach to bacterial biodiversity

This practical course aims to apply students' knowledge of microbiology and molecular biology to the identification of environmental bacteria.

Quantitative and qualitative analysis of the bacterial population present in a soil sample is classically done by identifying species using conventional bacteriological methods in successive stages: 1) isolation of bacterial flora; 2) diagnosis of family and genus using conventional media and tests; 3) diagnosis of species using API System galleries.

Molecular biology techniques now make it possible to identify the bacteria present in a sample without the need for cultivation. This approach requires access to a sequencing platform and will also be carried out as part of the practical work, enabling the two approaches to be compared. The sequencing results obtained will enable bioinformatic analysis of the rrsA gene specifying the RNA16S of isolated bacteria.

- Practical know-how in microbiology and molecular biology

- Make the most of your knowledge and develop your ability to analyze results

- Develop a critical and synthesizing mind

- Mobilize simple bioinformatics tools

basic knowledge of bacteriology and molecular biology