Biochemistry S3

This compulsory course enables students to consolidate the foundations of biochemistry acquired in L1 by approaching this discipline through a cross-disciplinary study of enzymes involved in cellular metabolism, particularly glycolysis. Several areas of biochemistry will be covered: the basics of Michael enzymology, description of the metabolic reactions involved in glycolysis. Finally, the technical aspect will be addressed through the presentation and analysis of techniques for measuring enzymatic activity, purifying, quantifying and detecting proteins.

Knowledge :

Understand the basic concepts of Michael enzymology and cellular energetics to understand metabolic regulation

Basic knowledge of carbohydrate structure and metabolism, particularly glycolysis.

Understand that the "spontaneity" of reactions constituting a metabolic pathway in cells is linked to a set of intracellular factors (varying concentrations of different metabolites over time) and extracellular factors (secreted metabolite concentrations, hormonal signals, etc.).

-Know-how :

Locate and measure enzymatic activity, measure reaction rate. Determine a metabolite.

Understand and use common spectrophotometric dosing and analysis techniques, and master common separation and purification methods.

Understanding inter-molecular interactions.

-People skills :

Working in pairs,

Use the workspace (benches and common work areas),

Communicating with teachers,

Respect the given working time,

Turn in work on time.

From Molecule to Cell (L1), Biochemistry and Cell Biology (L1)