Genome dynamics and expression

Lectures (57 hours)

 Review: From genes (DNA) to functional units of genes (proteins): genes, transcription, and translation (3 hours)

 DNA: enzymes for DNA manipulation, PCR, and cloning (6 hours)

 Genomes: genetic mapping, genome sequencing, genome annotation, gene function identification, prokaryotic genomes and eukaryotic organelles, nuclear eukaryotic genomes, viral genomes, and mobile genetic elements (12 hours)

 Gene expression: the role of DNA-binding proteins, transcriptional, post-transcriptional, translational, and post-translational regulation; methods for studying these different levels of regulation and protein-protein, protein-RNA, and protein-DNA interactions (15 hours)

 Gene expression in response to stress, during cell differentiation or development, epigenetics (9 hours)

 Genome replication, mutations and DNA repair, recombination, transposition, editing, and horizontal transfer (6 hours)

 How do genomes evolve? (6 hours)

 Practical work (24 hours)

 RNAseq analysis: RNA extraction, sequencing on the Bioenvironnement platform, differential expression analysis on Galaxy, enrichment analysis, validation of a few differentially expressed genes by RT-q-PCR.

Understanding the structure of genomes

Understanding how the information contained in a genome is used by the cell in which it resides. How genomes are expressed.

Understanding how genomes replicate and evolve

Knowledge of methods for studying genome structure, gene expression, and functional validation of genes

Continuous assessment: 100%