ECTS
10 credits
Component
Faculty of Science
Description
"Lectures (57 h)
Reminders: from genes (DNA) to functional units of genes (proteins): genes, transcription and translation (3H)
DNA: enzymes for DNA manipulation, PCR and cloning (6h)
Genomes: genetic mapping, genome sequencing, genome annotation, gene function identification, prokaryotic and eukaryotic organelle genomes, eukaryotic nuclear genomes, viral genomes and mobile genetic elements (12h)
Gene expression: the role of DNA-binding proteins, transcriptional, post-transcriptional, translational and post-translational regulation; methods for studying these different levels of regulation and protein-protein, protein-RNA, protein-DNA interactions (15h)
Gene expression in response to stress, during cell differentiation or development, epigenetics (9h)
Genome replication, mutations and DNA repair, recombination, transposition, editing and horizontal transfer (6h)
How genomes evolve (6h)
Practical work (24h)
RNAseq analysis: RNA extraction, sequencing on the Bioenvironnement platform, differential expression analysis on Galaxy, enrichment analysis, validation of some differentially expressed genes by RT-q-PCR".
Objectives
Understanding genome structure
How the information contained in a genome is used by the cell in which it resides. How genomes are expressed
Knowing how genomes replicate and evolve
Knowledge of methods for studying genome structure, expression and functional validation of genes
Knowledge control
100% continuous assessment