Genome Dynamics and Expression

"Lectures (57 hours)

 Review: From Genes (DNA) to the Functional Units of Genes (Proteins): Genes, Transcription, and Translation (3H)

 DNA: Enzymes for DNA Manipulation, PCR, and Cloning (6 hours)

 Genomes: genetic mapping, genome sequencing, genome annotation, gene function identification, prokaryotic genomes and eukaryotic organelle genomes, eukaryotic nuclear genomes, viral genomes, and mobile genetic elements (12 hours)

 Gene expression: the role of DNA-binding proteins; transcriptional, post-transcriptional, translational, and post-translational regulation; methods for studying these different levels of regulation and protein-protein, protein-RNA, and protein-DNA interactions (15 hours)

 Gene expression in response to stress, during cell differentiation or development, and epigenetics (9 hours)

 Genome replication, mutations and DNA repair, recombination, transposition, editing, and horizontal gene transfer (6 hours)

 How Do Genomes Evolve? (6 hours)

 Practical Work (24 hours)

 RNA-seq analysis: RNA extraction, sequencing on the Bioenvironnement platform, differential expression analysis on Galaxy, enrichment analysis, and validation of several differentially expressed genes by RT-q-PCR"

Understanding Genome Structure

Understanding how the information contained in a genome is used by the cell in which it resides. How genomes are expressed

Understanding how genomes replicate and evolve

Understand the methods used to study genome structure, gene expression, and the functional validation of genes

Comprehensive ongoing assessment: 100%