Protein Engineering

Proteins are now widely used as therapeutic tools in human and animal health. An understanding of the pathways involved in peptide and protein synthesis, their folding, and any post-translational modifications is essential before considering the biosynthesis of any therapeutic protein. Methods for better characterizing these proteins are also essential to ensure the quality of the proteins produced for therapeutic or industrial use. Methods of protein engineering designed to improve their original properties will also be discussed.

The EU program includes lectures and seminars led by faculty members and researchers.

The EU aims to provide students with knowledge about protein synthesis and structure, methods for studying proteins, and strategies for modifying them for industrial use. The concept of recombinant proteins will then be explored in greater depth in the M2 program.

By the end of the course, students will have acquired skills in:

-The structure and folding of proteins;

-Protein analysis methods: proteomics, mass spectrometry, structural analysis, structural bioinformatics;

-Protein translation and post-translational modifications;

-Peptide synthesis;

-The expression and purification of recombinant proteins;

-Protein engineering and its applications (vaccines, industrial enzymes, insulin, de novo proteins).

Students must be able to select the methods for isolating and analyzing peptides and proteins that are best suited to their project. They will be able to implement methods for modifying the structure and/or function of a protein for industrial applications.

A bachelor's degree (L3) in biochemistry and cell biology.

Assessment will be based on a final written exam.