Protein engineering

Proteins are now widely used as therapeutic tools in human and animal health. Knowledge of the synthesis pathways of peptides and proteins, their folding, and their possible post-translational modifications is essential before considering any biosynthesis of therapeutic proteins. Methods for better characterizing these proteins are also essential to guarantee the quality of proteins produced for therapeutic or industrial use. Protein engineering methods designed to improve their original properties will also be discussed.

The EU comprises lectures and tutorials, delivered by faculty members and researchers.

The EU aims to provide students with knowledge about protein synthesis and organization, methods for studying proteins, and strategies for modifying them for industrial use. The concept of recombinant proteins will then be explored in greater depth in M2.

At the end of the training, students will have acquired skills in:

-The structure and folding of proteins;

-Protein analysis methods: proteomics, mass spectrometry, structural analysis, structural bioinformatics;

-Translation and post-translational modifications of proteins;

-Peptide synthesis;

-The expression and purification of recombinant proteins;

-Protein engineering and its applications (vaccines, industrial enzymes, insulins, de novo proteins).

Students must be able to choose the methods for obtaining and analyzing peptides and proteins that are most suited to their project. They will be able to implement methods for modifying the structure and/or function of a protein for industrial application.

Bachelor's degree in biochemistry and cell biology.

Knowledge will be assessed by means of a final written exam.