Analysis of biomolecules by mass spectrometry

Knowledge of the latest mass spectrometry techniques for the qualitative analysis of organic molecules and biomolecules.

1) Description of fundamental principles (Ion science and technology):

- Ionization techniques

- Analysis techniques

- Tandem mass spectrometry (MS/MS)

- LC/MS and LC/MS/MS couplings

2) Application in the context of biomolecule analysis and monitoring of organic chemistry reactions.

Hourly volumes*:

CM: 3 p.m.

Tutorial: 5 hours

Be able to determine the mass spectrometry techniques suitable for analyzing an organic molecule and a biomolecule.

Understand and be able to analyze the strategy implemented to follow a given summary.

Mass spectrometry, Level 3:

Basic concepts (production/measurement of ions in the gas phase, instrument diagram)

Organic chemistry, Level 3:

Basic concepts of reactivity            

Biomolecule Chemistry, M1 level:

Knowledge of various families (structures, properties)

2-hour final written exam:

• Authorized documents: no
• Non-graphing calculator permitted: yes
• Internet access: no

Course: Inductive (problem-based) and deductive teaching methods, Materials available on the digital workspace (Moodle): Course documents, tutorial documents, past exam papers, and reference publications.

1. Review of fundamental principles: (3 hours)

- Production and transfer of ions in the gas phase,

- Sensitivity and detection limit,

- Measurement accuracy and resolution.

1. Ionization methods: (3 H)

- Ambient technologies,

- Desorption technologies.

1. Methods of analysis: (3 hours)

- Low-resolution analyzers,

- High-resolution analyzers.

1. Tandem mass spectrometry: (3 hours)

- Principle of MS/MS,

- Analyzer configuration (dissociations in space, time, iterative),

- Vibrational and electronic activation methods.

1. Coupled techniques: (3 hours)

- Principle of LC/MS,

- Principle of LC/MS/MS.

TD (5 hours): Individual work, exercises to be prepared before and during the session.

Case studies (interpretation of MS and MS/MS spectra of biomolecules, interpretation of LC/MS and LC/MS/MS data from reaction monitoring in organic chemistry/biomolecular chemistry).