Practical work in biochemistry

  • Level of study

    BAC +3

  • ECTS

    6 credits

  • Component

    Faculty of Science

Description

This course allows students to consolidate and deepen the practical management of numerous experimental data obtained during a week of practical work in a blocked period (5 consecutive days). These data are obtained following the elaboration of many different protocols, keeping in mind to ensure the best reproducibility of the preparations carried out and to have the best speed of execution in the preparation, the realization and the analysis of the various experiments. A large autonomy in the setting up of protocols will be encouraged, allowing in the long run experimental control and autonomy. This practical work also allows the management of group work (in pairs or in triads according to the capacity of reception and the number of students) and the writing of a report recording the protocols carried out, all the experimental data obtained and their analysis in order to determine a large set of biochemical parameters. A significant part of the evaluation will be based on the students' ability to generate, manage, exploit and analyze raw experimental data with the greatest rigor.

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Objectives

- Knowledge:

-Know how to set up and quickly develop a reliable experimental protocol.

-To master the methods of reagent preparation and the setting up of experiments in order to guarantee the best experimental reproducibility.

-Analyze, exploit and keep an objective eye on the relevance of raw data obtained.

-Know how to associate the results obtained with possible practical failures in order to improve the following experiments with relevance.

-To gain experimental autonomy.

-Know how to write a clear, precise, rigorous, analytical and critical experimental report.

-Know-how :

-Understand the experimental and practical constraints of obtaining a raw data set that must be exploited over a relatively long period of time (several consecutive days).

-Attaching the greatest rigor to the realization and exploitation of a diverse and varied set of data.

-Bring an objective and critical look at your results and conclusions.

-Know how to work in a team.

-Know how to be :

-Know how to elaborate independently a clear and rigorous experimental protocol.

-Know how to manage a large panel of experimental results by committing objectively to them.

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