Cellular and Molecular Biology 3

This compulsory UE of S4 allows students to consolidate and deepen the bases of molecular and cellular biology acquired in L1.

- Cell biology: The teaching will focus on 4 major themes: 1) The functioning of the cell cytoskeleton, 2) Cell adhesion, 3) Protein trafficking, 4) Introduction to cell cycle regulation. Cell biology methodologies will also be presented: immunoprecipitation for the detection of protein interactions, fluorescence videomicroscopy to follow the dynamics of cell distribution, evaluation of the importance of proteins of interest in a cellular process by strategies to modulate their expression (RNA interference, overexpression).

- Molecular biology : After acquiring in semester 3 knowledge concerning the mechanisms of transcription and translation, we will approach the regulation of gene expression: transcriptional regulation (repressor, activator) and attenuation in prokaryotes, the bases of the mechanisms of regulation of expression in eukaryotes.

- Knowledge:

Cell Biology:

-Understand and assimilate knowledge of molecular mechanisms related to the dynamic organization of the cytoskeleton, cell adhesion, vesicular trafficking and the cell cycle

-Understand the basic experimental approaches used in cell biology

Molecular biology: use the knowledge acquired in the previous semester to understand the main principles of the regulation of gene expression. Understand the experimental approaches to study these mechanisms

-Know-how :

Cell Biology:

Be able to establish molecular and functional links between the different chapters.

Be able to read and interpret results obtained by classical and varied experimental approaches used in cell biology.

Molecular Biology:

To be able to use the knowledge acquired in the previous semesters and during the biochemistry practical courses

Know how to interpret experimental results

-Know how to be :

Understand the different concepts presented and use them to be able to propose hypotheses on the functioning of molecular and cellular processes

Communicate with teachers and other students to carry out reflective exercises. Be able to formulate a hypothesis based on knowledge (in cell biology, biochemistry, molecular biology and physiology) and on experimental results obtained by basic approaches.

Be able to answer questions with clear and concise justification.

Be able to synthesize the functioning of a molecular mechanism in the form of a model diagram.

Molecular Biology 2 (HAV306V)